Protective effects of low-intensity pulsed ultrasound on aluminum-induced cerebral damage in Alzheimer's disease rat model

被引：164
作者：
Lin, Wei-Ting [1 ]
Chen, Ran-Chou [1 ,4 ]
Lu, Wen-Wei [1 ]
Liu, Shing-Hwa [3 ]
Yang, Feng-Yi [1 ,2 ]
机构：
[1] Natl Yang Ming Univ, Dept Biomed Imaging & Radiol Sci, Taipei 112, Taiwan
[2] Natl Yang Ming Univ, Biophoton & Mol Imaging Res Ctr, Taipei 112, Taiwan
[3] Natl Taiwan Univ, Coll Med, Inst Toxicol, Taipei 10764, Taiwan
[4] Taipei City Hosp, Dept Radiol, Taipei, Taiwan
来源：
SCIENTIFIC REPORTS | 2015年 / 5卷
关键词：
BRAIN-BARRIER DISRUPTION; NEUROTROPHIC FACTOR; FOCUSED ULTRASOUND; DELIVERY; BDNF; MEMORY; MOUSE; DYSFUNCTION; MECHANISMS; GENE;
D O I：
10.1038/srep09671
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The protein expressions of neurotrophic factors can be enhanced by low-intensity pulsed ultrasound (LIPUS) stimulation in the brain. The purpose of this study was to demonstrate the protective effect of LIPUS stimulation against aluminum-induced cerebral damage in Alzheimer's disease rat model. LIPUS was administered 7 days before each aluminum chloride (AlCl3) administration, and concomitantly given with AlCl3 daily for a period of 6 weeks. Neurotrophic factors in hippocampus were measured by western blot analysis. Behavioral changes in the Morris water maze and elevated plus maze were examined in rats after administration of AlCl3. Various biochemical analyses were performed to evaluate the extent of brain damages. LIPUS is capable of prompting levels of brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), and vascular endothelial growth factor (VEGF) in rat brain. AlCl3 administration resulted in a significant increase in the aluminum concentration, acetylcholinesterase activity and beta-amyloid (Ab) deposition in AlCl3 treated rats. LIPUS stimulation significantly attenuated aluminum concentration, acetylcholinesterase activity, Ab deposition and karyopyknosis in AlCl3 treated rats. Furthermore, LIPUS significantly improved memory retention in AlCl3-induced memory impairment. These experimental results indicate that LIPUS has neuroprotective effects against AlCl3-induced cerebral damages and cognitive dysfunction.
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页数：7
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