Rapid and Accurate Molecular Identification of the Emerging Multidrug-Resistant Pathogen Candida auris

被引:149
作者
Kordalewska, Milena [1 ]
Zhao, Yanan [1 ]
Lockhart, Shawn R. [2 ]
Chowdhary, Anuradha [3 ]
Berrio, Indira [4 ,5 ,6 ]
Perlin, David S. [1 ]
机构
[1] Rutgers Biomed & Hlth Sci, Publ Hlth Res Inst, Newark, NJ 07103 USA
[2] Ctr Dis Control & Prevent, Mycot Dis Branch, Atlanta, GA USA
[3] Univ Delhi, Vallabhbhai Patel Chest Inst, Dept Med Mycol, Delhi, India
[4] Clin El Rosario, Medellin, Colombia
[5] Corp Invest Biol CIB, Med & Expt Mycol Grp, Medellin, Colombia
[6] Hosp Gen Medellin Luz Castro Gutierrez ESE, Medellin, Colombia
关键词
Candida duobushaemulonii; Candida haemulonii; Candida lusitaniae; Candida auris; PCR; diagnostics; identification; real-time PCR; DESORPTION IONIZATION-TIME; ANTIFUNGAL SUSCEPTIBILITY; NOSOCOMIAL FUNGEMIA; CLONAL STRAIN; HAEMULONII; EMERGENCE;
D O I
10.1128/JCM.00630-17
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Candida auris is an emerging multidrug-resistant fungal pathogen causing nosocomial and invasive infections associated with high mortality. C. auris is commonly misidentified as several different yeast species by commercially available phenotypic identification platforms. Thus, there is an urgent need for a reliable diagnostic method. In this paper, we present fast, robust, easy-to-perform and interpret PCR and real-time PCR assays to identify C. auris and related species: Candida duo-bushaemulonii, Candida haemulonii, and Candida lusitaniae. Targeting rDNA region nucleotide sequences, primers specific for C. auris only or C. auris and related species were designed. A panel of 140 clinical fungal isolates was used in both PCR and real-time PCR assays followed by electrophoresis or melting temperature analysis, respectively. The identification results from the assays were 100% concordant with DNA sequencing results. These molecular assays overcome the deficiencies of existing phenotypic tests to identify C. auris and related species.
引用
收藏
页码:2445 / 2452
页数:8
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