Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats

被引：3

作者：

Araujo, Juscilania Furtado ^{[1
]}

Andrioli, Alice ^{[2
]}

Pinheiro, Raymundo Rizaldo ^{[2
]}

Peixoto, Renato Mesquita ^{[2
,3
]}

de Sousa, Ana Lidia Madeira ^{[4
]}

de Azevedo, Dalva Alana Aragao ^{[5
]}

Lima, Ana Milena Cesar ^{[6
]}

Nobre, Juliana Araujo ^{[7
]}

Amaral, Gabriel Paula ^{[8
]}

Brandao, Iane Sousa ^{[9
]}

Teixeira, Maria Fatima da Silva ^{[10
]}

机构：

[1] Univ Estadual Ceara, Northeast Network Biotechnol, Fortaleza, Ceara, Brazil

[2] Embrapa Goats & Sheep, Sobral, Ceara, Brazil

[3] Natl Council Sci & Technol Dev DCR CNPq FUNCAP, Scholarship Reg Sci Dev, Level C, Brasilia, DF, Brazil

[4] Embrapa Pigs & Poultry, Concordia, SC, Brazil

[5] Univ Estadual Ceara, Vet Sci, Fortaleza, Ceara, Brazil

[6] Univ Fed Piaui, Anim Sci, Teresina, Piaui, Brazil

[7] Univ Fed Ceara, Med Microbiol, Fortaleza, Ceara, Brazil

[8] State Univ Acarau Valley, Zootech, Sobral, Ceara, Brazil

[9] State Univ Acarau Valley, Biol Sci, Sobral, Ceara, Brazil

[10] Univ Estadual Ceara, Fortaleza, Ceara, Brazil

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2021年 / 78卷

关键词：

Amniotic sac; Goats; SRLVs; Retrovirus; Intrauterine transmission; ARTHRITIS-ENCEPHALITIS VIRUS; MAEDI-VISNA VIRUS; CAPRINE LENTIVIRUS; IN-VITRO; TRANSMISSION; CELLS; RNA; INFECTION; COLOSTRUM; OOCYTES;

D O I：

10.1016/j.cimid.2021.101693

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative. The amniotic fluid was collected from each of the eight animals during cesarean section at 147 days of pregnancy. Cocultivation was undertaken using secondary goat nictitating membrane cell cultures obtained by explant from a small ruminant lentivirus-negative calf followed by trypsinization and sub-cultivation of the cells for 63 days. During this period, five supernatant collections were performed for DNA extraction and subsequent nested polymerase chain reaction. DNA was extracted from the amniotic fluid after 3 h of cellular sedimentation, from which a sample of 600 ILL was taken from the sediment and another 600 ILL sample from the supernatant. After DNA extraction, nested polymerase chain reaction was performed. Of the eight goats, 62.5 % (05/08) were small ruminant lentivirus-positive, with 43.75 % (07/16) of the total samples positive when considering the two repetitions (supernatant and cell sediment). Moreover, positivity was confirmed by small ruminant lentivirus pro-viral DNA amplification in the cell supernatant throughout the cocultivation period. Small ruminant lentivirus were present in the amniotic fluid samples from the naturally infected goats indicating an intrauterine transmission route. Moreover, this biological fluid can be adopted for the diagnosis of these lentiviruse because it is an important risk factor related to intrauterine transmission.

引用

页数：6