Pre-mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa

被引：13

作者：

Mendes, Niege S. ^{[1
]}

Silva, Patricia M. ^{[1
]}

Silva-Rocha, Rafael ^{[2
]}

Martinez-Rossi, Nilce M. ^{[1
]}

Rossi, Antonio ^{[1
]}

机构：

[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP, Brazil

[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Mol & Cellular Biol, BR-14049900 Ribeirao Preto, SP, Brazil

来源：

FEBS OPEN BIO | 2016年 / 6卷 / 04期

基金：

巴西圣保罗研究基金会;

关键词：

alternative splicing; amphotericin B; asparagine synthetase; intron retention; ketoconazole; Neurospora crassa; Pi regulation; TRANSCRIPTIONAL ACTIVATOR NUC-1; ENDOPLASMIC-RETICULUM STRESS; PHOSPHATE STARVATION; DISK DIFFUSION; MICRODILUTION METHODS; ASPERGILLUS-NIDULANS; MAJOR REGULATOR; GENETIC-CONTROL; MUTANT STRAIN; FLUFFY GENE;

D O I：

10.1002/2211-5463.12047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

For this study, we sought to identify pre-mRNA processing events modulated by changes in extracellular pH, inorganic phosphate, and antifungal drugs. We examined genes with at least four putative introns whose transcriptional level responded to these effectors. We showed that the intron retention levels of genes encoding asparagine synthetase 2, C6-zinc finger regulator (fluffy), and a farnesyltransferase respond to amphotericin B, ketoconazole, and other effectors. In general, the assayed antifungals promoted the disruption of the structural domains of these proteins probably leading to their inactivation, which emphasize the complexity of the metabolic modulation exerted by antifungal signaling.

引用

页码：358 / 368

页数：11

共 50 条

[21]

Lenburg ME, 1996, TRENDS BIOCHEM SCI, V21, P383, DOI 10.1016/S0968-0004(96)10048-7

[22] NEUROSPORA-CRASSA MUTANTS DEFICIENT IN ASPARAGINE SYNTHETASE [J].

MACPHEE, KG ;

NELSON, RE ;

SCHUSTER, SM .

JOURNAL OF BACTERIOLOGY, 1983, 156 (01) :475-478

[23] DEFECTIVE SPLICING OF MITOCHONDRIAL RIBOSOMAL-RNA IN CYTOCHROME-DEFICIENT NUCLEAR MUTANTS OF NEUROSPORA-CRASSA [J].

MANNELLA, CA ;

COLLINS, RA ;

GREEN, MR ;

LAMBOWITZ, AM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (06) :2635-2639

[24] The fungal genetics stock center: From molds to molecules [J].

McCluskey, K .

ADVANCES IN APPLIED MICROBIOLOGY, VOL 52, 2003, 52 :245-262

[25] The geranylgeranyl diphosphate synthase gene of Gibberella fujikuroi: Isolation and expression [J].

Mende, K ;

Homann, V ;

Tudzynski, B .

MOLECULAR AND GENERAL GENETICS, 1997, 255 (01) :96-105

[26] Correlation between microdilution, Etest, and disk diffusion methods for antifungal susceptibility testing of fluconazole against Candida sp blood isolates [J].

Menezes, Everardo Albuquerque ;

de Vasconcelos Junior, Antonio Alexandre ;

Ferreira Angelo, Maria Rozzele ;

dos Santos Oliveira Cunha, Maria da Conceicao ;

Cunha, Francisco Afranio .

REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL, 2013, 46 (01) :106-107

[27]

METZENBERG RL, 1979, GENETICS, V93, P625

[28] Comparison between disk diffusion and microdilution methods for determining susceptibility of clinical fungal isolates to caspofungin [J].

Milici, Maria Eleonora ;

Maida, Carmelo Massimo ;

Spreghini, Elisabetta ;

Ravazzolo, Barbara ;

Oliveri, Salvatore ;

Scalise, Giorgio ;

Barchiesi, Francesco .

JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (11) :3529-3533

[29] HacA-Dependent Transcriptional Switch Releases hacA mRNA from a Translational Block upon Endoplasmic Reticulum Stress [J].

Mulder, Harm J. ;

Nikolaev, Igor .

EUKARYOTIC CELL, 2009, 8 (04) :665-675

[30] Antifungal susceptibility of candida species using the clinical and laboratory standards institute disk diffusion and broth microdilution methods [J].

Noake, T. ;

Kuriyama, T. ;

White, P. L. ;

Potts, Aj. C. ;

Lewis, M. A. O. ;

Williams, D. W. ;

Barnes, R. A. .

JOURNAL OF CHEMOTHERAPY, 2007, 19 (03) :283-287

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