Pre-mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa

被引：13
作者：
Mendes, Niege S. [1 ]
Silva, Patricia M. [1 ]
Silva-Rocha, Rafael [2 ]
Martinez-Rossi, Nilce M. [1 ]
Rossi, Antonio [1 ]
机构：
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto, SP, Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Mol & Cellular Biol, BR-14049900 Ribeirao Preto, SP, Brazil
来源：
FEBS OPEN BIO | 2016年 / 6卷 / 04期
基金：
巴西圣保罗研究基金会;
关键词：
alternative splicing; amphotericin B; asparagine synthetase; intron retention; ketoconazole; Neurospora crassa; Pi regulation; TRANSCRIPTIONAL ACTIVATOR NUC-1; ENDOPLASMIC-RETICULUM STRESS; PHOSPHATE STARVATION; DISK DIFFUSION; MICRODILUTION METHODS; ASPERGILLUS-NIDULANS; MAJOR REGULATOR; GENETIC-CONTROL; MUTANT STRAIN; FLUFFY GENE;
D O I：
10.1002/2211-5463.12047
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
For this study, we sought to identify pre-mRNA processing events modulated by changes in extracellular pH, inorganic phosphate, and antifungal drugs. We examined genes with at least four putative introns whose transcriptional level responded to these effectors. We showed that the intron retention levels of genes encoding asparagine synthetase 2, C6-zinc finger regulator (fluffy), and a farnesyltransferase respond to amphotericin B, ketoconazole, and other effectors. In general, the assayed antifungals promoted the disruption of the structural domains of these proteins probably leading to their inactivation, which emphasize the complexity of the metabolic modulation exerted by antifungal signaling.
引用
收藏
页码：358 / 368
页数：11
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