Long non-coding RNA UCA1 promotes papillary thyroid cancer cell proliferation via miR-204-mediated BRD4 activation

Long non-coding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) has been used in tumor development and progression in many types of cancer. However, the function and mechanism underlying the action of UCA1 in papillary thyroid cancer (PTC) remains unclear. Therefore, these topics were investigated in the present study by in vitro and in vivo experiments. It was demonstrated that the expression level of UCA1 was more significantly upregulated in PTC cell lines and tissues when compared with the immortal human thyroid follicular cell line and adjacent normal tissues, respectively. UCA1 knockdown significantly inhibited PTC cell viability, colony formation and the bromodomain containing 4 (BRD4) expression level in vitro, and retarded FTC tumor growth in vivo. In the previous study, microRNA (miR)-204 inhibited thyroid cancer progression and was regulated by UCA1 in other types of cancer. In addition, by conducting dual luciferase reporter assays, it was confirmed that miR-204 directly binds to UCA1 and the 3'-untranslated region of BRD4. Furthermore, UCA1 competed with BRD4 for miR-204 binding. miR-204 knockdown enhanced BRD4 expression, which can be partially restored by short hairpin-UCA1. The results of the present study illustrated that UCA1 promotes PTC progression by acting as a competing endogenous RNA by sponging miR-204. In conclusion, UCA1 may be regarded as an oncogenic lncRNA, promoting FTC cell proliferation, and be a potential target for human FTC treatment.