Characterization of Reversible Associations by Sedimentation Velocity with UltraScan

被引：39

作者：

Demeler, Borries ^{[1
]}

Brookes, Emre ^{[1
]}

Wang, Renjing ^{[1
]}

Schirf, Virgil ^{[1
]}

Kim, Chongwoo A. ^{[1
]}

机构：

[1] Univ Texas Hlth Sci Ctr San Antonio, Dept Biochem, San Antonio, TX 78229 USA

来源：

MACROMOLECULAR BIOSCIENCE | 2010年 / 10卷 / 07期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

analytical ultracentrifugation; monomers; reversible associations; equilibrium complexes and rate constants; RING1B; polycomb repression complex 1; sedimentation velocity; structure; ultrascan; ANALYTICAL ULTRACENTRIFUGATION EXPERIMENTS; FINITE-ELEMENT SOLUTION;

D O I：

10.1002/mabi.200900481

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We compare here the utility of sedimentation velocity (SV) to sedimentation equilibrium (SE) analysis for the characterization of reversible systems. Genetic algorithm optimization in Ultra Scan is used to optimize the model and to obtain solution properties of all components present in the system. We apply our method to synthetic and experimental data, and suggest limits for the accessible kinetic range. We conclude that equilibrium constants obtained from SV and SE analysis are equivalent, but that SV experiments provide better confidence for the K-d, can better account for the presence of contaminants and provide additional information including rate constants and shape parameters.

引用

页码：775 / 782

页数：8

共 23 条

[1] Detection of Protein Aggregates by Sedimentation Velocity Analytical Ultracentrifugation (SV-AUC): Sources of Variability and Their Relative Importance [J].