Induction of MafBx and Murf ubiquitin ligase mRNAs in rat skeletal muscle after LPS injection

被引：123
作者：
Dehoux, MJM [1 ]
van Beneden, RP [1 ]
Fernández-Celemín, L [1 ]
Lause, PL [1 ]
Thissen, JPM [1 ]
机构：
[1] Univ Catholique Louvain, Unite Diabetol & Nutr, B-1200 Brussels, Belgium
来源：
FEBS LETTERS | 2003年 / 544卷 / 1-3期
关键词：
muscle; ubiquitin ligase; tumor necrosis factor-alpha; insulin like growth factor-1; lipopolysaccharide; real-time quantitative polymerase chain reaction;
D O I：
10.1016/S0014-5793(03)00505-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
MafBx and Murf are two new rat E3 ubiquitin ligases induced in muscle atrophy. Our goal was to investigate whether lipopolysaccharide (LPS) injection, a model of muscle catabolism, is associated with increased expression of MafBx and Murf. LPS (750 mug/100 g body weight) induces MafBx and Murf mRNA (respectively, 23-fold and 33-fold after 12 h; P < 0.001). A transient induction of tumor necrosis factor-alpha mRNA (21-fold; P < 0.001 at 3 h) and a decrease of insulin like growth factor-I mRNA (50%; P < 0.001 at 6 h), two potential regulators of the ubiquitin-proteasome system were also demonstrated. In summary, MafBx and Murf mRNA are upregulated in response to LPS and might play a role in the muscle proteolysis observed. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码：214 / 217
页数：4
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