MiRNA profile of osteosarcoma with CD117 and stro-1 expression: miR-1247 functions as an onco-miRNA by targeting MAP3K9

被引：0

作者：

Zhao, Fuyou ^{[1
]}

Lv, Jie ^{[4
]}

Gan, Huaiyong ^{[2
]}

Li, Yumei ^{[1
]}

Wang, Ri ^{[1
]}

Zhang, Haoran ^{[1
]}

Wu, Qiong ^{[1
]}

Chen, Yuqing ^{[3
]}

机构：

[1] Bengbu Med Coll, Affiliated Hosp 1, Dept Med Oncol, Bengbu 233000, Anhui, Peoples R China

[2] Bengbu Med Coll, Affiliated Hosp 1, Dept Pathol, Bengbu 233000, Anhui, Peoples R China

[3] Bengbu Med Coll, Affiliated Hosp 1, Dept Resp Dis, Bengbu 233000, Anhui, Peoples R China

[4] Bengbu Med Coll, Dept Microbiol, Bengbu 233000, Anhui, Peoples R China

来源：

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY | 2015年 / 8卷 / 02期

关键词：

miR-1247; miRNA profile; osteosarcoma; MAP3K9; STEM-CELLS; DIFFERENTIATION;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

microRNAs (miRNA) are regulators of gene expression, but little is known about miRNA expression profiles in stem cells of osteosarcoma (OS). C117 and Stro-1 are known stem cell markers of OS. In the study, CD117 and stro-1 positive (CD117(+)stro-1(+)) and CD117 and stro-1 negative (CD117 stro-1) cells were isolated from MG63 cells CD117(+)stro-1(+) cells showed more metastatic ability and stem cell formation rate than CD117 stro-1 ones. To find the difference between CD117(+) stro-1(+) and CD117 stro-1 cells, the miRNA expression profile was examined using DNA microarray. MicroRNAs were differentially expressed in osteosarcoma cells with CD117(+) stro-1(+) and CD117(-) stro-1. The significant miRNAs included miR-15a, miR-302a, miR-423-5p, miR-1247, miR-1243 and others, which were confirmed by real time RT-PCR. The significant down-regulated miR-1247 was confirmed that was a potential tumor suppressor by targeting MAP3K9. Our results indicated that dysregulation of miRNAs is involved in osteosarcoma and miR-1247 plays an important role in progression of osteosarcoma.

引用

页码：1451 / 1458

页数：8