SiRNA Directed Against Annexin II Receptor Inhibits Angiogenesis via Suppressing MMP2 and MMP9 Expression

被引：25

作者：

Song, Hongyuan ^{[1
]}

Pan, Dongyan ^{[1
]}

Sun, Weifeng ^{[1
]}

Gu, Cao ^{[1
]}

Zhang, Yuelu ^{[1
]}

Zhao, Ping ^{[2
]}

Qi, Zhongtian ^{[2
]}

Zhao, Shihong ^{[1
]}

机构：

[1] Second Mil Univ, Changhai Hosp, Dept Ophthalmol, Shanghai 200433, Peoples R China

[2] Second Mil Univ, Dept Microbiol, Shanghai Key Lab Med Biodef, Shanghai 200433, Peoples R China

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2015年 / 35卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Annexin II receptor; C5orf39; Angiogenesis; Matrix metalloproteinase; siRNA; NF-KAPPA-B; MATRIX METALLOPROTEINASES; APOPTOSIS; GROWTH; MIGRATION; ADHESION; INVASION; SYSTEM;

D O I：

10.1159/000369745

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: Annexin II receptor (AXIIR) is able to mediate Annexin II signal and induce apoptosis, but its role in angiogenesis remains unclear. This study tries to investigate the role of AXIIR in angiogenesis and the plausible molecular mechanism. Methods/Results: RNA interference technology was used to silence AXIIR, and the subsequent effects in vitro and in vivo were evaluated thereafter. Our data indicated that human umbilical vein endothelial cells (HUVECs) expressed AXIIR and knockdown of AXIIR significantly inhibited HUVECs proliferation, adhesion, migration, and tube formation in vitro and suppressed angiogenesis in vivo. Furthermore, AXIIR siRNA induced cell arrest in the S/G2 phase while had no effect on cell apoptosis. We found that these subsequent effects might be via suppressing the expression of matrix metalloproteinase 2and matrix metalloproteinase 9. Conclusion: AXIIR participates in angiogenesis, and may be a potential therapeutic target for angiogenesis related diseases. Copyright (C) 2015 S. Karger AG, Basel

引用

页码：875 / 884

页数：10

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