Helicobacter pylori heat shock protein B (HspB) localizes in vivo in the gastric mucosa and MALT lymphoma

被引：9

作者：

De Luca, Antonio ^{[1
]}

De Falco, Maria ^{[2
]}

Manente, Lucrezia ^{[3
]}

Dattilo, Doriana ^{[1
]}

Lucariello, Angela ^{[1
]}

Esposito, Vincenzo ^{[4
]}

Gnarini, Mariarosaria ^{[4
]}

Citro, Gennaro ^{[5
]}

Baldi, Alfonso ^{[6
]}

Tufano, Maria Antonietta ^{[3
]}

Iaquinto, Gaetano ^{[7
]}

机构：

[1] Univ Naples 2, Dept Med & Publ Hlth, I-80138 Naples, Italy

[2] Univ Naples Federico II, Dept Biol Sci, Sect Evolutionary & Comparat Biol, Naples, Italy

[3] Univ Naples 2, Dept Expt Med, I-80138 Naples, Italy

[4] III Div AO D Cotugno, Naples, Italy

[5] Regina Elena Inst Canc Res, SAFU Dept, Rome, Italy

[6] Univ Naples 2, Dept Biochem & Biophys F Cedrangolo, Sect Anat Pathol, I-80138 Naples, Italy

[7] San G Moscati Hosp, Div Gastroenterol, Avellino, Italy

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2008年 / 216卷 / 01期

关键词：

D O I：

10.1002/jcp.21376

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Heat shock protein B (HspB) is one of the dominant proteins recognized by most Helicobacter pylori-infected persons and is being considered as potential candidates for subunit vaccines. In the present study we describe the generation of an antibody against HspB and its use in immunohistochemical assays on gastric biopsies. We have demonstrated that our rabbit polyclonal antibody against HspB did not recognize any protein in lysates from a lung human epithelial cell (H1299) line and did not cross-react with the other members of human heat shock proteins. Secondly, we have observed that in gastric biopsies, HspB immunostaining was present inside the cytoplasm of human epithelial cells with a particular localization in the apical portion of gastric epithelial cells other than in the extracellular spaces among gastric cells of human stomach. Finally, we have demonstrated a cytoplasmic HspB immunostaining in groups of neoplastic cells of MALT lymphoma. In conclusion, our observations suggest a possible involvement of HspB in the pathogenesis of H. pylori-related pathologies such as gastritis, ulcer and gastric cancer.

引用

页码：78 / 82

页数：5

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