Depletion of Intracellular Zinc Inhibits the Ubiquitin Ligase Activity of Viral Regulatory Protein ICP0 and Restricts Herpes Simplex Virus 1 Replication in Cell Culture

被引：13

作者：

Grant, Kyle ^{[1
]}

Grant, Louise ^{[1
]}

Tong, Lily ^{[1
]}

Boutell, Chris ^{[1
]}

机构：

[1] Univ Glasgow, MRC, Ctr Virus Res, Glasgow, Lanark, Scotland

来源：

JOURNAL OF VIROLOGY | 2012年 / 86卷 / 07期

基金：

英国医学研究理事会;

关键词：

RING FINGER DOMAIN; BINDING DOMAIN; TYPE-1; MUTANT; IN-VITRO; PML; INFECTION; VMW110; GENE; DEGRADATION; EXPRESSION;

D O I：

10.1128/JVI.06962-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The viral ubiquitin ligase ICP0 stimulates the onset of HSV-1 lytic infection and productive reactivation of viral genomes from latency. In order to mediate these processes, it requires its C3HC4 RING finger domain, a tertiary structural fold that is coordinated by the binding of two zinc (Zn2+) atoms. Here we formally demonstrate that Zn2+ binding and intracellular Zn2+ levels are critical for ICP0's biochemical activity and that depletion of intracellular Zn2+ severely attenuates HSV-1 replication.

引用

页码：4029 / 4033

页数：5

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