In this study, the use of low percentages of biodiesel blends from different feedstock's was investigated because it leads to little level performance decrease, with biodiesel advantages on reducing pollutant emissions according to ULSD when used in diesel engines only with a small amount of carbon residue in the combustion chamber. Therefore, firstly, biodiesel fuels were obtained from unique resources of animal, vegetable and microalgae oils by transesterification method. Then these biodiesel fuels were blended with ULSD by 10% and were named as here AOB10, VOB10 and MOB10, respectively. Afterword, these biodiesel blends were compared with ULSD by testing the combustion, performance and emission in a diesel engine generator under 3.52 kW, 6.98 kW and 10.37 kW loads with constant speed of 1500 rpm. According to the results, for all engine operating loads although the BSFC values of blends were higher compared to the ULSD, all blends gave almost identical results. In addition, blends had identical values of CGP, CHR, HRR, knock density and AGT compared to ULSD operation in engine. In the cases of usages of all blends; smoke opacity, HC and CO emissions considerably decreased, while NOx and CO2 slightly increased in all loads when compared to ULSD. (C) 2020 Elsevier Ltd. All rights reserved.