Aging alters neuronal nitric oxide release from rat mesenteric arteries:: role of presynaptic β-adrenoceptors

被引:15
作者
Ferrer, M [1 ]
Balfagón, G [1 ]
机构
[1] Univ Autonoma Madrid, Fac Med, Dept Fisiol, E-28029 Madrid, Spain
关键词
adrenergic innervation; beta-adrenoceptors; aging; electrical field stimulation; nitrergic innervation; rat mesenteric artery;
D O I
10.1042/CS20010029
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
This study examines the influence of aging on the neuronal nitric oxide (NO) and noradrenaline (NA) release elicited by electrical field stimulation (EFS; 200 mA, 0.3 ms, 1-16 Hz, 30 s) in endothelium-denuded mesenteric arteries from young and old rats, as well as the influence of the presynaptic beta -adrenoceptors in that release, EFS induced frequency-dependent contractions. N-G-nitro-L-arginine methyl ester (L-NAME) only enhanced EFS-elicited contractions in arteries from young rats. Capsaicin did not alter the EFS-induced contractions in either age group. Clenbuterol did not modify the contraction elicited by EFS in arteries from young or old rats either. A subsequent addition of L-NAME also induced an increase in the EFS-induced response in arteries from both age groups. In old rats, the presence of propranolol did not alter the response induced by EFS, and the subsequent addition of clenbuterol or clenbuterol plus L-NAME did not affect this response. In precontracted segments, sodium nitroprusside or clenbuterol induced similar relaxation in both age groups. None of the drugs used altered the response to exogenous NA or basal tone. In arteries preincubated with [H-3]NA, EFS induced H-3 release, which remained unmodified in the presence of clenbuterol or propranolol in young rats, whereas clenbuterol increased the H-3 overflow in old rats, and this effect was abolished by propranolol. These drugs did not alter the basal H-3 efflux and indicate that in rat mesenteric arteries EFS induces NA release in both age groups, and only NO release in young animals. Activation of presynaptic beta -adrenoceptors increased NA and probably NO release in aged rats.
引用
收藏
页码:321 / 328
页数:8
相关论文
共 47 条
  • [1] ARRIBAS S, 1994, J PHARMACOL EXP THER, V270, P520
  • [2] Nitric oxide and the non-adrenergic non-cholinergic neurotransmission
    Boeckxstaens, GE
    Pelckmans, PA
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 1997, 118 (04): : 925 - 937
  • [3] BUCHHOLZ J, 1990, J PHARMACOL EXP THER, V252, P159
  • [4] Effects of aging on prejunctional control of neurotransmission in the rat
    Docherty, JR
    [J]. PHARMACOLOGICAL INTERVENTION IN AGING AND AGE-ASSOCIATED DISORDERS: PROCEEDINGS OF THE SIXTH CONGRESS OF THE INTERNATIONAL ASSOCIATION OF BIOMEDICAL GERONTOLOGY, 1996, 786 : 264 - 273
  • [5] DOCHERTY JR, 1990, PHARMACOL REV, V42, P103
  • [6] ALPHA-ADRENOCEPTOR RESPONSIVENESS IN THE AGED RAT
    DOCHERTY, JR
    HYLAND, L
    [J]. EUROPEAN JOURNAL OF PHARMACOLOGY, 1986, 126 (1-2) : 75 - 80
  • [7] DUCKLES SP, 1982, BRAIN RES, V245, P171
  • [8] Effect of clenbuterol on the modulation of noradrenaline release in the rat tail artery
    Encabo, A
    Ferrer, M
    Salaices, M
    Manso, R
    Marin, J
    Balfagon, G
    [J]. JOURNAL OF AUTONOMIC PHARMACOLOGY, 1996, 16 (05): : 243 - 250
  • [9] LOCAL NADPH DIAPHORASE NEURONS INNERVATE PIAL-ARTERIES AND LIE CLOSE OR PROJECT TO INTRACEREBRAL BLOOD-VESSELS - A POSSIBLE ROLE FOR NITRIC-OXIDE IN THE REGULATION OF CEREBRAL BLOOD-FLOW
    ESTRADA, C
    MENGUAL, E
    GONZALEZ, C
    [J]. JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1993, 13 (06) : 978 - 984
  • [10] FERNANDES F, 1992, J HYPERTENS S4, V10, pS65