Association of endothelial dysfunction with endothelin, nitric oxide and eNOS Glu298Asp gene polymorphism in coronary artery disease

被引：1

作者：

Saini, Vandana ^{[1
]}

Bhatnagar, M. K. ^{[2
]}

Bhattacharjee, Jayashree ^{[2
]}

机构：

[1] Lady Hardinge Med Coll & Associated Hosp, Dept Biochem, New Delhi, India

[2] Lady Hardinge Med Coll & Associated Hosp, Dept Med, New Delhi, India

来源：

DISEASE MARKERS | 2011年 / 31卷 / 04期

关键词：

Nitric oxide; Coronary artery disease; Glu298Asp polymorphism; endothelin; SYNTHASE GENE; RISK-FACTOR; COMMON VARIANT; EXON; 7; ATHEROSCLEROSIS; EXPRESSION;

D O I：

10.1155/2011/419708

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The endothelial dysfunction has been implicated as a major event in the pathogenesis of atherosclerosis. Therefore, this study was planned to determine (a) role of endothelium-derived nitric oxide (NO) and endothelin as coronary artery disease (CAD) risk markers and (b) intergenotypic variation of endothelial nitric oxide synthase (eNOS) Glu298Asp polymorphism in CAD. The endothelin, NO and eNOS genotypes were determined in 60 patients with documented history of CAD. These were compared with 50 age- and sex- matched healthy controls. The genotype frequencies for eNOS gene polymorphism were determined by PCR and RFLP. The plasma endothelin in CAD patients was significantly higher (p < 0.001) whereas, the NO level in CAD group was significantly lower (p < 0.001) than the control group. The genotype frequencies for Glu298/Asp (Glu/Glu and Glu/Asp) genotypes were 75% and 25% in CAD subjects and 88% and 12% in control subjects, respectively. No Asp/Asp was found in any of the groups. The genotype frequencies differed significantly (p < 0.05) between the controls and cases. In conclusion, endothelin and NO may be used as markers of endothelial dysfunction in CAD. Asp allele might be a risk factor for CAD in the North Indian population.

引用

页码：215 / 222

页数：8

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