Isolation and characterization of polymorphic microsatellites from red coral grouper (Plectropomus maculatus)

被引：16

作者：

Zhu, ZY ^{[1
]}

Lo, LC ^{[1
]}

Lin, G ^{[1
]}

Xu, YX ^{[1
]}

Yue, GH ^{[1
]}

机构：

[1] Natl Univ Singapore, Mol Populat Genet Grp, Temasek Life Sci Lab, Singapore 117548, Singapore

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 03期

关键词：

cross-amplification; grouper; microsatellite; Serranidae;

D O I：

10.1111/j.1471-8286.2005.00995.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The red coral grouper, Plectropomus maculatus, is a high value marine food fish species. We isolated the first set of 10 polymorphic microsatellites and examined their polymorphism in two related species: the Malabar grouper Epinephelus malabaricus and the brown-marbled grouper Epinephelus fuscoguttatus. The average allele number of these 10 microsatellite DNA loci is 9.1/locus with a range of four to 17, whereas the expected heterozygosity ranged from 0.18 to 0.94, averaging 0.76. Five of the 10 markers amplified polymorphic and specific products in E. malabaricus, whereas only four were amplified in E. fuscoguttatus. These microsatellites could be applicable to captive breeding and to the studies on genetic diversity and population structure of grouper wild stocks.

引用

页码：579 / 581

页数：3

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