Amperometric Biosensor for Choline Based on Gold Screen-Printed Electrode Modified with Electrochemically-Deposited Silica Biocomposite

被引：19
|
作者：
Mazurenko, I. [1 ,3 ,4 ]
Tananaiko, O. [1 ]
Biloivan, O. [2 ]
Zhybak, M. [2 ]
Pelyak, I. [1 ]
Zaitsev, V. [1 ,5 ]
Etienne, M. [3 ,4 ]
Walcarius, A. [3 ,4 ]
机构：
[1] Taras Shevchenko Natl Univ Kyiv, Dept Analyt Chem, UA-01033 Kiev, Ukraine
[2] NAS Ukraine, Lab Biomol Elect, Inst Mol Biol & Genet, UA-03143 Kiev, Ukraine
[3] Univ Lorraine, F-54600 Villers Les Nancy, France
[4] CNRS, UMR 7564, Lab Chim Phys & Microbiol Environm, F-54600 Villers Les Nancy, France
[5] Pontificia Univ Catolica Rio de Janeiro, Dept Chem, BR-22451900 Rio De Janeiro, Brazil
来源：
ELECTROANALYSIS | 2015年 / 27卷 / 07期
关键词：
Amperometric biosensor; Choline oxidase; Screen-printed electrode; Sol-gel; Electrochemically-assisted deposition; PRUSSIAN BLUE; THIN-FILMS; ENZYME ELECTRODE; OXIDASE; NANOPARTICLES; GLUCOSE; ACETYLCHOLINE; ENCAPSULATION; SENSORS; IMMOBILIZATION;
D O I：
10.1002/elan.201400694
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A mediator-free choline biosensor was developed using the electrochemically assisted sol-gel deposition on gold screen-printed electrodes. The addition of 12 mM of cationic surfactant CTAB in silica sol allowed enhancing the stability of the sensor. The modified electrode demonstrated catalytic activity and stable amperometric response to choline for over 3 weeks of exploitation with the sensitivity of 6 mu AmM1 and LOD of 6 mu M. The interference of ascorbic acid was reduced by pretreating the analyzed solution with MnO2 powder. The application of the sensor with the purpose of identifying choline in the baby milk demonstrated satisfactory metrological characteristics.
引用
收藏
页码：1685 / 1692
页数：8
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