Confocal Imaging at the Nanoscale with Two-Color STED Microscopy

被引：0
作者：
Gugel, Hilmar [1 ]
Giske, Arnold [1 ]
Dyba, Marcus [1 ]
Sieber, Jochen [1 ]
机构：
[1] Leica Microsyst CMS, D-68165 Mannheim, Germany
来源：
SINGLE MOLECULE SPECTROSCOPY AND IMAGING IV | 2011年 / 7905卷
关键词：
STED; Confocal; Microscopy; Two-color; Imaging; Superresolution; Nanoscopy; FLUORESCENCE MICROSCOPY; STIMULATED-EMISSION; RESOLUTION; LIMIT;
D O I：
10.1117/12.874779
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
STED microscopy enables confocal imaging of biological samples with a resolution that is not limited by diffraction. It provides new insights in various fields of biology, such as membrane biology, neurobiology and physiology. Its three dimensional sectioning ability allows the acquisition of high resolution image stacks. Furthermore, STED microscopy enables the recording of dynamic processes and live cell images. We present two-color imaging in confocal STED microscopy with a single STED wavelength. Pulsed and continuous wave lasers in the visible and near infra-red wavelengths range are used for stimulated emission. The resolution enhancement is demonstrated in comparison to confocal imaging with biological specimens.
引用
收藏
页数：7
相关论文
共 15 条
[1]   Imaging intracellular fluorescent proteins at nanometer resolution [J].
Betzig, Eric ;
Patterson, George H. ;
Sougrat, Rachid ;
Lindwasser, O. Wolf ;
Olenych, Scott ;
Bonifacino, Juan S. ;
Davidson, Michael W. ;
Lippincott-Schwartz, Jennifer ;
Hess, Harald F. .
SCIENCE, 2006, 313 (5793) :1642-1645
[2]   Two-color far-field fluorescence nanoscopy [J].
Donnert, Gerald ;
Keller, Jan ;
Wurm, Christian A. ;
Rizzoli, Silvio O. ;
Westphal, Volker ;
Schoenle, Andreas ;
Jahn, Reinhard ;
Jakobs, Stefan ;
Eggeling, Christian ;
Hell, Stefan W. .
BIOPHYSICAL JOURNAL, 2007, 92 (08) :L67-L69
[3]  
GISKE A, 2010, P SPIE, V7578
[4]  
Hell S., 2009, SINGLE MOL SPECTROSC, P365
[5]   Toward fluorescence nanoscopy [J].
Hell, SW .
NATURE BIOTECHNOLOGY, 2003, 21 (11) :1347-1355
[6]   BREAKING THE DIFFRACTION RESOLUTION LIMIT BY STIMULATED-EMISSION - STIMULATED-EMISSION-DEPLETION FLUORESCENCE MICROSCOPY [J].
HELL, SW ;
WICHMANN, J .
OPTICS LETTERS, 1994, 19 (11) :780-782
[7]   Ultra-high resolution imaging by fluorescence photoactivation localization microscopy [J].
Hess, Samuel T. ;
Girirajan, Thanu P. K. ;
Mason, Michael D. .
BIOPHYSICAL JOURNAL, 2006, 91 (11) :4258-4272
[8]   Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission [J].
Klar, TA ;
Jakobs, S ;
Dyba, M ;
Egner, A ;
Hell, SW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (15) :8206-8210
[9]   Dual-color STED microscopy at 30-nm focal-plane resolution [J].
Meyer, Lars ;
Wildanger, Dominik ;
Medda, Rebecca ;
Punge, Annedore ;
Rizzoli, Silvio O. ;
Donnert, Gerald ;
Hell, Stefan W. .
SMALL, 2008, 4 (08) :1095-1100
[10]   Fast STED microscopy with continuous wave fiber lasers [J].
Moneron, Gael ;
Medda, Rebecca ;
Hein, Birka ;
Giske, Arnold ;
Westphal, Volker ;
Hell, Stefan W. .
OPTICS EXPRESS, 2010, 18 (02) :1302-1309
12