Modulation of Flavivirus Population Diversity by RNA Interference

被引：33

作者：

Brackney, Doug E. ^{[1
]}

Schirtzinger, Erin E. ^{[2
]}

Harrison, Thomas D. ^{[1
]}

Ebel, Gregory D. ^{[1
]}

Hanley, Kathryn A. ^{[2
]}

机构：

[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Arthropod Borne & Infect Dis Lab, Ft Collins, CO 80523 USA

[2] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA

来源：

JOURNAL OF VIROLOGY | 2015年 / 89卷 / 07期

基金：

美国国家卫生研究院;

关键词：

WEST-NILE-VIRUS; ANTIVIRAL IMMUNITY; INNATE IMMUNITY; DROSOPHILA; ESCAPE; INFECTION; CELLS; MOSQUITOS; ALPHAVIRUS; SELECTION;

D O I：

10.1128/JVI.02612-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To test the hypothesis that RNA interference (RNAi) imposes diversifying selection on RNA virus genomes, we quantified West Nile virus (WNV) quasispecies diversity after passage in Drosophila cells in which RNAi was left intact, depleted, or stimulated against WNV. As predicted, WNV diversity was significantly lower in RNAi-depleted cells and significantly greater in RNAi-stimulated cells relative to that in controls. These findings reveal that an innate immune defense can shape viral population structure.

引用

页码：4035 / 4039

页数：5

共 36 条

[1] Comparison of siRNA-induced off-target RNA and protein effects
Aleman, Lourdes M.
Doench, John
Sharp, Phillip A.
[J]. RNA, 2007, 13 (03) : 385 - 395
[2] Human immunodeficiency virus type 1 escape from RNA interference
Boden, D
Pusch, O
Lee, F
Tucker, L
Ramratnam, B
[J]. JOURNAL OF VIROLOGY, 2003, 77 (21) : 11531 - 11535
[3] Replication of Many Human Viruses Is Refractory to Inhibition by Endogenous Cellular MicroRNAs
Bogerd, Hal P.
Skalsky, Rebecca L.
Kennedy, Edward M.
Furuse, Yuki
Whisnant, Adam W.
Flores, Omar
Schultz, Kimberly L. W.
Putnam, Nicole
Barrows, Nicholas J.
Sherry, Barbara
Scholle, Frank
Garcia-Blanco, Mariano A.
Griffin, Diane E.
Cullen, Bryan R.
[J]. JOURNAL OF VIROLOGY, 2014, 88 (14) : 8065 - 8076
[4] RNAi Targeting of West Nile Virus in Mosquito Midguts Promotes Virus Diversification
Brackney, Doug E.
Beane, Jennifer E.
Ebel, Gregory D.
[J]. PLOS PATHOGENS, 2009, 5 (07)
[5] Epitope-Specific CD8+ T Lymphocytes Cross-Recognize Mutant Simian Immunodeficiency Virus (SIV) Sequences but Fail To Contain Very Early Evolution and Eventual Fixation of Epitope Escape Mutations during SIV Infection
Cale, Evan M.
Hraber, Peter
Giorgi, Elena E.
Fischer, Will
Bhattacharya, Tanmoy
Leitner, Thomas
Yeh, Wendy W.
Gleasner, Cheryl
Green, Lance D.
Han, Cliff S.
Korber, Bette
Letvin, Norman L.
[J]. JOURNAL OF VIROLOGY, 2011, 85 (08) : 3746 - 3757
[6] West Nile virus infection of Drosophila melanogaster induces a protective RNAi response
Chotkowski, Heather L.
Ciota, Alexander T.
Jia, Yongqing
Puig-Basagoiti, Francesc
Kramer, Laura D.
Shi, Pei-Yong
Glaser, Robert L.
[J]. VIROLOGY, 2008, 377 (01) : 197 - 206
[7] Is RNA Interference a Physiologically Relevant Innate Antiviral Immune Response in Mammals?
Cullen, Bryan R.
Cherry, Sara
tenOever, Benjamin R.
[J]. CELL HOST & MICROBE, 2013, 14 (04) : 374 - 378
[8] The DExD/H-box helicase Dicer-2 mediates the induction of antiviral activity in drosophila
Deddouche, Safia
Matt, Nicolas
Budd, Aidan
Mueller, Stefanie
Kemp, Cordula
Galiana-Arnoux, Delphine
Dostert, Catherine
Antoniewski, Christophe
Hoffmann, Jules A.
Imler, Jean-Luc
[J]. NATURE IMMUNOLOGY, 2008, 9 (12) : 1425 - 1432
[9] The outcome of hepatitis C virus infection is predicted by escape mutations in epitopes targeted by cytotoxic T lymphocytes
Erickson, AL
Kimura, Y
Igarashi, S
Eichelberger, J
Houghton, M
Sidney, J
McKinney, D
Sette, A
Hughes, AL
Walker, CM
[J]. IMMUNITY, 2001, 15 (06) : 883 - 895
[10] Transport of dsRNA into cells by the transmembrane protein SID-1
Feinberg, EH
Hunter, CP
[J]. SCIENCE, 2003, 301 (5639) : 1545 - 1547

← 1 2 3 4 →