Modulation of Flavivirus Population Diversity by RNA Interference

被引：33

作者：

Brackney, Doug E. ^{[1
]}

Schirtzinger, Erin E. ^{[2
]}

Harrison, Thomas D. ^{[1
]}

Ebel, Gregory D. ^{[1
]}

Hanley, Kathryn A. ^{[2
]}

机构：

[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Arthropod Borne & Infect Dis Lab, Ft Collins, CO 80523 USA

[2] New Mexico State Univ, Dept Biol, Las Cruces, NM 88003 USA

来源：

JOURNAL OF VIROLOGY | 2015年 / 89卷 / 07期

基金：

美国国家卫生研究院;

关键词：

WEST-NILE-VIRUS; ANTIVIRAL IMMUNITY; INNATE IMMUNITY; DROSOPHILA; ESCAPE; INFECTION; CELLS; MOSQUITOS; ALPHAVIRUS; SELECTION;

D O I：

10.1128/JVI.02612-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To test the hypothesis that RNA interference (RNAi) imposes diversifying selection on RNA virus genomes, we quantified West Nile virus (WNV) quasispecies diversity after passage in Drosophila cells in which RNAi was left intact, depleted, or stimulated against WNV. As predicted, WNV diversity was significantly lower in RNAi-depleted cells and significantly greater in RNAi-stimulated cells relative to that in controls. These findings reveal that an innate immune defense can shape viral population structure.

引用

页码：4035 / 4039

页数：5

共 36 条

[1] Comparison of siRNA-induced off-target RNA and protein effects [J].

Aleman, Lourdes M. ;

Doench, John ;

Sharp, Phillip A. .

RNA, 2007, 13 (03) :385-395

[2] Human immunodeficiency virus type 1 escape from RNA interference [J].

Boden, D ;

Pusch, O ;

Lee, F ;

Tucker, L ;

Ramratnam, B .

JOURNAL OF VIROLOGY, 2003, 77 (21) :11531-11535

[3] Replication of Many Human Viruses Is Refractory to Inhibition by Endogenous Cellular MicroRNAs [J].

Bogerd, Hal P. ;

Skalsky, Rebecca L. ;

Kennedy, Edward M. ;

Furuse, Yuki ;

Whisnant, Adam W. ;

Flores, Omar ;

Schultz, Kimberly L. W. ;

Putnam, Nicole ;

Barrows, Nicholas J. ;

Sherry, Barbara ;

Scholle, Frank ;

Garcia-Blanco, Mariano A. ;

Griffin, Diane E. ;

Cullen, Bryan R. .

JOURNAL OF VIROLOGY, 2014, 88 (14) :8065-8076

[4] RNAi Targeting of West Nile Virus in Mosquito Midguts Promotes Virus Diversification [J].

Brackney, Doug E. ;

Beane, Jennifer E. ;

Ebel, Gregory D. .

PLOS PATHOGENS, 2009, 5 (07)

[5] Epitope-Specific CD8+ T Lymphocytes Cross-Recognize Mutant Simian Immunodeficiency Virus (SIV) Sequences but Fail To Contain Very Early Evolution and Eventual Fixation of Epitope Escape Mutations during SIV Infection [J].

Cale, Evan M. ;

Hraber, Peter ;

Giorgi, Elena E. ;

Fischer, Will ;

Bhattacharya, Tanmoy ;

Leitner, Thomas ;

Yeh, Wendy W. ;

Gleasner, Cheryl ;

Green, Lance D. ;

Han, Cliff S. ;

Korber, Bette ;

Letvin, Norman L. .

JOURNAL OF VIROLOGY, 2011, 85 (08) :3746-3757

[6] West Nile virus infection of Drosophila melanogaster induces a protective RNAi response [J].

Chotkowski, Heather L. ;

Ciota, Alexander T. ;

Jia, Yongqing ;

Puig-Basagoiti, Francesc ;

Kramer, Laura D. ;

Shi, Pei-Yong ;

Glaser, Robert L. .

VIROLOGY, 2008, 377 (01) :197-206

[7] Is RNA Interference a Physiologically Relevant Innate Antiviral Immune Response in Mammals? [J].

Cullen, Bryan R. ;

Cherry, Sara ;

tenOever, Benjamin R. .

CELL HOST & MICROBE, 2013, 14 (04) :374-378

[8] The DExD/H-box helicase Dicer-2 mediates the induction of antiviral activity in drosophila [J].

Deddouche, Safia ;

Matt, Nicolas ;

Budd, Aidan ;

Mueller, Stefanie ;

Kemp, Cordula ;

Galiana-Arnoux, Delphine ;

Dostert, Catherine ;

Antoniewski, Christophe ;

Hoffmann, Jules A. ;

Imler, Jean-Luc .

NATURE IMMUNOLOGY, 2008, 9 (12) :1425-1432

[9] The outcome of hepatitis C virus infection is predicted by escape mutations in epitopes targeted by cytotoxic T lymphocytes [J].

Erickson, AL ;

Kimura, Y ;

Igarashi, S ;

Eichelberger, J ;

Houghton, M ;

Sidney, J ;

McKinney, D ;

Sette, A ;

Hughes, AL ;

Walker, CM .

IMMUNITY, 2001, 15 (06) :883-895

[10] Transport of dsRNA into cells by the transmembrane protein SID-1 [J].

Feinberg, EH ;

Hunter, CP .

SCIENCE, 2003, 301 (5639) :1545-1547

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