Liquid Chromatography-Mass Spectrometry-based Quantitative Proteomics

被引：141

作者：

Xie, Fang ^{[1
]}

Liu, Tao ^{[1
]}

Qian, Wei-Jun ^{[1
]}

Petyuk, Vladislav A. ^{[1
]}

Smith, Richard D. ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2011年 / 286卷 / 29期

基金：

美国能源部; 美国国家卫生研究院;

关键词：

COMPLEX PROTEIN MIXTURES; ABSOLUTE QUANTIFICATION; SIGNALING NETWORKS; ACCURATE MASS; IN-VIVO; EXPRESSION PROTEOMICS; PEPTIDES; STRATEGY; PLASMA; TAGS;

D O I：

10.1074/jbc.R110.199703

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

LC-MS-based quantitative proteomics has become increasingly applied to a wide range of biological applications due to growing capabilities for broad proteome coverage and good accuracy and precision in quantification. Herein, we review the current LC-MS-based quantification methods with respect to their advantages and limitations and highlight their potential applications.

引用

页码：25443 / 25449

页数：7

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