Liquid Chromatography-Mass Spectrometry-based Quantitative Proteomics

被引:141
作者
Xie, Fang [1 ]
Liu, Tao [1 ]
Qian, Wei-Jun [1 ]
Petyuk, Vladislav A. [1 ]
Smith, Richard D. [1 ]
机构
[1] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA
基金
美国能源部; 美国国家卫生研究院;
关键词
COMPLEX PROTEIN MIXTURES; ABSOLUTE QUANTIFICATION; SIGNALING NETWORKS; ACCURATE MASS; IN-VIVO; EXPRESSION PROTEOMICS; PEPTIDES; STRATEGY; PLASMA; TAGS;
D O I
10.1074/jbc.R110.199703
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
LC-MS-based quantitative proteomics has become increasingly applied to a wide range of biological applications due to growing capabilities for broad proteome coverage and good accuracy and precision in quantification. Herein, we review the current LC-MS-based quantification methods with respect to their advantages and limitations and highlight their potential applications.
引用
收藏
页码:25443 / 25449
页数:7
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