Liquid Chromatography-Mass Spectrometry-based Quantitative Proteomics

被引：137
|
作者：
Xie, Fang [1 ]
Liu, Tao [1 ]
Qian, Wei-Jun [1 ]
Petyuk, Vladislav A. [1 ]
Smith, Richard D. [1 ]
机构：
[1] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA
来源：
JOURNAL OF BIOLOGICAL CHEMISTRY | 2011年 / 286卷 / 29期
基金：
美国能源部; 美国国家卫生研究院;
关键词：
COMPLEX PROTEIN MIXTURES; ABSOLUTE QUANTIFICATION; SIGNALING NETWORKS; ACCURATE MASS; IN-VIVO; EXPRESSION PROTEOMICS; PEPTIDES; STRATEGY; PLASMA; TAGS;
D O I：
10.1074/jbc.R110.199703
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
LC-MS-based quantitative proteomics has become increasingly applied to a wide range of biological applications due to growing capabilities for broad proteome coverage and good accuracy and precision in quantification. Herein, we review the current LC-MS-based quantification methods with respect to their advantages and limitations and highlight their potential applications.
引用
收藏
页码：25443 / 25449
页数：7
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