Magnetic-field-enabled resolution enhancement in super-resolution imaging

被引：6

作者：

Zhang, Min ^{[1
,2
]}

Chen, Junling ^{[1
,2
]}

Gao, Jing ^{[1
,2
]}

Wang, Zhiyong ^{[1
]}

Xu, Haijiao ^{[1
]}

Cai, Mingjun ^{[1
]}

Jiang, Junguang ^{[1
]}

Tian, Zhiyuan ^{[3
]}

Wang, Hongda ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Univ Chinese Acad Sci, Sch Chem & Chem Engn, Beijing 100049, Peoples R China

来源：

PHYSICAL CHEMISTRY CHEMICAL PHYSICS | 2015年 / 17卷 / 10期

关键词：

LOCALIZATION ANALYSIS; FLUORESCENCE; MICROSCOPY; PHOTOSTABILITY; RECOMBINATION; FLUOROPHORES; TRACKING;

D O I：

10.1039/c4cp05914j

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A novel strategy for modulating the photophysics of organic dyes in super-resolution fluorescence imaging using an external magnetic field was reported. The magnetic field induced increase in fluorescence intensity, localization number of probe molecules, and the number of photons emitted per molecule as compared to those acquired without a magnetic field were experimentally confirmed. Improved dSTORM localization precision and imaging resolution were consequently achieved.

引用

页码：6722 / 6727

页数：6

共 36 条

[1] An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments [J].

Aitken, Colin Echeverria ;

Marshall, R. Andrew ;

Puglisi, Joseph D. .

BIOPHYSICAL JOURNAL, 2008, 94 (05) :1826-1835

[2] Magnetic field effect on fluorescence in a mixture of N-ethylcarbazole and dimethyl terephthalate in a polymer film in tine presence of electric fields [J].

Awasthi, Kamlesh ;

Mizoguchi, Miwako ;

Iimori, Toshifumi ;

Nakabayashi, Takakazu ;

Ohta, Nobuhiro .

JOURNAL OF PHYSICAL CHEMISTRY A, 2008, 112 (19) :4432-4436

[3] Multicolor super-resolution imaging with photo-switchable fluorescent probes [J].

Bates, Mark ;

Huang, Bo ;

Dempsey, Graham T. ;

Zhuang, Xiaowei .

SCIENCE, 2007, 317 (5845) :1749-1753

[4] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[5] Photoswitching Mechanism of Cyanine Dyes [J].

Dempsey, Graham T. ;

Bates, Mark ;

Kowtoniuk, Walter E. ;

Liu, David R. ;

Tsien, Roger Y. ;

Zhuang, Xiaowei .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (51) :18192-+

[6] BIOCHEMISTRY WITHOUT OXYGEN [J].

ENGLANDER, SW ;

CALHOUN, DB ;

ENGLANDER, JJ .

ANALYTICAL BIOCHEMISTRY, 1987, 161 (02) :300-306

[7] Fluorescence nanoscopy by ground-state depletion and single-molecule return [J].

Foelling, Jonas ;

Bossi, Mariano ;

Bock, Hannes ;

Medda, Rebecca ;

Wurm, Christian A. ;

Hein, Birka ;

Jakobs, Stefan ;

Eggeling, Christian ;

Hell, Stefan W. .

NATURE METHODS, 2008, 5 (11) :943-945

[8] Simple Method to Enhance the Photostability of the Fluorescence Reporter R6G for Prolonged Single-Molecule Studies [J].

Guo, Lin ;

Gai, Feng .

JOURNAL OF PHYSICAL CHEMISTRY A, 2013, 117 (29) :6164-6170

[9] Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution [J].

Gustafsson, MGL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (37) :13081-13086

[10] Photophysics of Fluorescent Probes for Single-Molecule Biophysics and Super-Resolution Imaging [J].

Ha, Taekjip ;

Tinnefeld, Philip .

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 63, 2012, 63 :595-617

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