A protocol for rapid pericyte differentiation of human induced pluripotent stem cells

被引：14

作者：

Aisenbrey, Elizabeth A. ^{[1
]}

Torr, Elizabeth ^{[2
]}

Johnson, Hunter ^{[3
]}

Soref, Cheryl ^{[4
]}

Daly, William ^{[2
]}

Murphy, William L. ^{[2
,3
]}

机构：

[1] Univ Wisconsin, Dept Surg, Madison, WI 53705 USA

[2] Univ Wisconsin, Dept Orthoped, Madison, WI 53705 USA

[3] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53705 USA

[4] Univ Wisconsin, Waisman Ctr, Madison, WI 53705 USA

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 01期

基金：

美国国家卫生研究院;

关键词：

ENDOTHELIAL-CELLS; PROGENITOR CELLS; EXPRESSION; INDUCTION; MARKER; CD34;

D O I：

10.1016/j.xpro.2020.100261

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pericytes play a critical role in promoting, regulating, and maintaining numerous vascular functions. Their dysfunction is a major contributor to the progression of vascular and neurodegenerative diseases, making them an ideal candidate for large-scale production for disease modeling and regenerative cell therapy. This protocol describes the rapid and robust differentiation of pericytes from human induced pluripotent stem cells (hiPSCs) while simultaneously generating a population of hiPSC-derived endothelial progenitor cells. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2017).

引用

页数：15

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