Alpha C protein-specific immunity in humans with group B streptococcal colonization and invasive disease

被引:11
作者
Pannaraj, Pia S. [1 ,2 ]
Kelly, Joanna K. [1 ,2 ]
Rench, Marcia A. [1 ,2 ]
Madoff, Lawrence C. [3 ,4 ]
Edwards, Morven S. [1 ,2 ]
Baker, Carol J. [1 ,2 ,5 ,6 ]
机构
[1] Baylor Coll Med, Dept Pediat, Infect Dis Sect, Houston, TX 77030 USA
[2] Texas Childrens Hosp, Dept Pediat, Infect Dis Sect, Houston, TX 77030 USA
[3] Harvard Univ, Sch Med, Brigham & Womens Hosp, Channing Lab, Boston, MA 02115 USA
[4] Harvard Univ, Sch Med, Brigham & Womens Hosp, Div Infect Dis, Boston, MA 02115 USA
[5] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA
[6] Texas Childrens Hosp, Dept Mol Virol & Microbiol, Houston, TX 77030 USA
关键词
group B Streptococcus; alpha C protein; conjugate vaccine;
D O I
10.1016/j.vaccine.2007.11.034
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Alpha C protein, found in 76% of non-type III strains of group B Streptococcus (GBS), elicits antibodies protective against alpha C-expressing strains in experimental animals, making it an appealing carrier for a GBS conjugate vaccine. We determined whether natural exposure to alpha C elicits antibodies in women. Geometric mean concentrations of alpha C-specific IgM and IgG were similar by ELISA in sera from 58 alpha C GBS strain colonized and 174 age-matched non-colonized women (IgG 245 and 313 ng/ml; IgM 257 and 229 ng/ml, respectively), but acute sera from 13 women with invasive alpha C-expressing GBS infection had significantly higher concentrations (IgM 383 and IgG 476 ng/ml [p=0.036 and 0.038, respectively]). Convalescent sera from 5 of these women 16-49 days later had high alpha C-specific IgM and IgG concentrations (1355 and 4173 ng/ml, respectively). In vitro killing of alpha C-expressing GBS correlated with total alpha C-specific antibody concentration. Invasive disease but not colonization elicits alpha C-specific IgM and IgG in adults. (C) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码:502 / 508
页数:7
相关论文
共 31 条
[1]   CORRELATION OF MATERNAL ANTIBODY DEFICIENCY WITH SUSCEPTIBILITY TO NEONATAL GROUP-B STREPTOCOCCAL INFECTION [J].
BAKER, CJ ;
KASPER, DL .
NEW ENGLAND JOURNAL OF MEDICINE, 1976, 294 (14) :753-756
[2]   Identification of a glycosaminoglycan binding region of the alpha c protein that mediates entry of group B streptococci into host cells [J].
Baron, Miriam J. ;
Filman, David J. ;
Prophete, Gina A. ;
Hogle, James M. ;
Madoff, Lawrence C. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (14) :10526-10536
[3]   Alpha C protein of group B Streptococcus binds host cell surface glycosaminoglycan and enters cells by an actin-dependent mechanism [J].
Baron, MJ ;
Bolduc, GR ;
Goldberg, MB ;
Aupérin, TC ;
Madoff, LC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (23) :24714-24723
[4]   Improved methods for typing nontypeable isolates of group B streptococci [J].
Benson, JA ;
Flores, AE ;
Baker, CJ ;
Hillier, SL ;
Ferrieri, P .
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2002, 292 (01) :37-42
[5]  
BEVANGER L, 1985, ACTA PATH MICRO IM B, V93, P121
[6]  
BEVANGER L, 1985, ACTA PATH MICRO IM B, V93, P113
[7]   Group B streptococcal colonization and serotype-specific immunity in pregnant women at delivery [J].
Campbell, JR ;
Hillier, SL ;
Krohn, MA ;
Ferrieri, P ;
Zaleznik, DF ;
Baker, CJ .
OBSTETRICS AND GYNECOLOGY, 2000, 96 (04) :498-503
[8]  
*CDCP, 2005, MMWR-MORBID MORTAL W, V54, P1205
[9]   The frequency of genes encoding three putative group B streptococcal virulence factors among invasive and colonizing isolates [J].
D Manning, Shannon ;
Ki, Moran ;
Marrs, Carl F. ;
Kugeler, Kiersten J. ;
Borchardt, Stephanie M. ;
Baker, Carol J. ;
Foxman, Betsy .
BMC INFECTIOUS DISEASES, 2006, 6 (1)
[10]   Antibodies to capsular polysaccharides of group B Streptococcus in pregnant Canadian women:: Relationship to colonization status and infection in the neonate [J].
Davies, HD ;
Adair, C ;
McGeer, A ;
Ma, D ;
Robertson, S ;
Mucenski, M ;
Kowalsky, L ;
Tyrell, G ;
Baker, CJ .
JOURNAL OF INFECTIOUS DISEASES, 2001, 184 (03) :285-291