Purification of oligouronides by immobilized L-histidine pseudoaffinity chromatography

被引：13
作者：
Delattre, C. [1 ]
Kamalanathan, A. S. [1 ]
Michaud, P. [2 ]
Vijayalakshmi, M. A. [1 ]
机构：
[1] Univ Vellore, Vellore Inst Technol, Ctr BioSeparatTechnol, Vellore 632014, Tamil Nadu, India
[2] Univ Blaise Pascal, Polytech Clermont Ferrand, Lab Genie Chim & Biochim, F-63174 Aubiere, France
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2008年 / 861卷 / 02期
关键词：
oligouronides; purification; pseudobioaffinity; L-histidine; monolith chromatography; CIM-disk;
D O I：
10.1016/j.jchromb.2007.06.024
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Selective purification of alpha-(1,4)-oligogalacturonides was investigated using several pseudobioaffinity chromatography matrix with aminoacid L-histidine as pseudobiospecific ligand: (1) sepharose 4B-bisoxyran-histidine, (2) sepharose 4B-epoxy-histidine, (3) silica-oxyran-histidine and (4) CIM-disk-EDA-histidine. These anionic oligosaccharides prepared by enzymatic and chemical cleavage of polygalacturonic acid were used as models sugar in order to optimize the adsorption and elution parameters for a selective purification of bioactive oligouronides. Monolithic CIM-disk chromatography is one of the fastest liquid chromatographic method using for separation and purification of biomolecules thanks to high mass transfer rate. In this way, this new monolithic CIM-disk system with L-histidine immobilized: immobilized histidine affinity chromatography (IHAC) constitutes a good tool allowing the fast and selective purification of bioactive oligouronides. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码：181 / 185
页数：5
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