Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

被引：12

作者：

Nylk, J. ^{[1
,2
]}

Kristensen, M. V. G. ^{[1
]}

Mazilu, M. ^{[1
]}

Thayil, A. K. ^{[1
,2
]}

Mitchell, C. A. ^{[1
,2
]}

Campbell, E. C. ^{[2
]}

Powis, S. J. ^{[3
]}

Gunn-Moore, F. J. ^{[2
]}

Dholakia, K. ^{[1
]}

机构：

[1] Univ St Andrews, Sch Phys & Astron, SUPA, St Andrews KY16 9SS, Fife, Scotland

[2] Univ St Andrews, Sch Biol, St Andrews KY16 9TF, Fife, Scotland

[3] Univ St Andrews, Sch Med, St Andrews KY16 9TF, Fife, Scotland

来源：

BIOMEDICAL OPTICS EXPRESS | 2015年 / 6卷 / 04期

基金：

英国工程与自然科学研究理事会;

关键词：

TWEEZERS; MICROSCOPY; CELL; MANIPULATION; SYNAPSES;

D O I：

10.1364/BOE.6.001512

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200 mu m from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation. (C) 2015 Optical Society of America

引用

页码：1512 / 1519

页数：8

共 24 条

[1] Power spectrum analysis for optical tweezers
Berg-Sorensen, K
Flyvbjerg, H
[J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 2004, 75 (03) : 594 - 612
[2] Adiabatic submicrometric tapers for optical tweezers
Brambilla, G.
Xu, F.
[J]. ELECTRONICS LETTERS, 2007, 43 (04) : 204 - 206
[3] Shaping the light transmission through a multimode optical fibre: complex transformation analysis and applications in biophotonics
Cizmar, Tomas
Dholakia, Kishan
[J]. OPTICS EXPRESS, 2011, 19 (20): : 18871 - 18884
[4] Optical micromanipulation
Dholakia, Kishan
Reece, Peter
Gu, Min
[J]. CHEMICAL SOCIETY REVIEWS, 2008, 37 (01) : 42 - 55
[5] Optical manipulation in combination with multiphoton microscopy for single-cell studies
Goksör, M
Enger, J
Hanstorp, D
[J]. APPLIED OPTICS, 2004, 43 (25) : 4831 - 4837
[6] Graded-index optical fiber tweezers with long manipulation length
Gong, Yuan
Huang, Wei
Liu, Qun-Feng
Wu, Yu
Rao, Yunjiang
Peng, Gang-Ding
Lang, Jinyi
Zhang, Ke
[J]. OPTICS EXPRESS, 2014, 22 (21): : 25267 - 25276
[7] Graded-index fiber tip optical tweezers: Numerical simulation and trapping experiment
Gong, Yuan
Ye, Ai-Yan
Wu, Yu
Rao, Yun-Jiang
Yao, Yao
Xiao, Song
[J]. OPTICS EXPRESS, 2013, 21 (13): : 16181 - 16190
[8] The Immunological Synapse: A Molecular Machine Controlling T Cell Activation
Grakoui, Arash
Bromley, Shannon K.
Sumen, Cenk
Davis, Mark M.
Shaw, Andrey S.
Allen, Paul M.
Dustin, Michael L.
[J]. JOURNAL OF IMMUNOLOGY, 2015, 194 (09) : 221 - 227
[9] Efficient subpixel image registration algorithms
Guizar-Sicairos, Manuel
Thurman, Samuel T.
Fienup, James R.
[J]. OPTICS LETTERS, 2008, 33 (02) : 156 - 158
[10] Heller I, 2013, NAT METHODS, V10, P910, DOI [10.1038/NMETH.2599, 10.1038/nmeth.2599]

← 1 2 3 →