Molecular Characterization of Tomato Leaf Curl China Virus, Infecting Tomato Plants in China, and Functional Analyses of Its Associated Betasatellite

被引：28

作者：

Yang, Xiuling ^{[1
]}

Guo, Wei ^{[1
]}

Ma, Xinying ^{[1
]}

An, Qianli ^{[1
]}

Zhou, Xueping ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310029, Peoples R China

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 09期

基金：

中国国家自然科学基金;

关键词：

CUCUMBER-MOSAIC-VIRUS; DNA-BETA SATELLITE; SEQUENCE ALIGNMENT; GENE-EXPRESSION; BEGOMOVIRUS; DISEASE; PATHOGENICITY; PROTEIN; RECOMBINATION; YUNNAN;

D O I：

10.1128/AEM.00017-11

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel tomato-infecting begomovirus from Guangxi province, China, was identified and characterized, for which the name Tomato leaf curl China virus (ToLCCNV) was proposed. Phylogenetic and recombination analyses of the virus genomic sequences suggested that ToLCCNV may have arisen by recombination among Tomato leaf curl Vietnam virus (ToLCVV), Tomato leaf curl Gujarat virus (ToLCGV), and an unknown virus. A betasatellite molecule was found to be associated with ToLCCNV (ToLCCNB), and its complete nucleotide sequences were determined. Infectious clones of ToLCCNV and ToLCCNB were constructed and then used for agro-inoculation of plants; ToLCCNV alone infected Nicotiana benthamiana, Nicotiana glutinosa, Petunia hybrida, and Solanum lycopersicum plants, but no symptoms were induced. ToLCCNB was required for induction of leaf curl disease in these hosts. The beta C1 protein of ToLCCNB was identified as a suppressor of RNA silencing and accumulated primarily in the nucleus. Deletion mutagenesis of beta C1 showed that the central part of beta C1 (amino acids 44 to 74) was responsible for both the suppressor activity and nuclear localization.

引用

页码：3092 / 3101

页数：10

共 42 条

[1] Recommendations for the classification and nomenclature of the DNA-β satellites of begomoviruses [J].

Briddon, R. W. ;

Brown, J. K. ;

Moriones, E. ;

Stanley, J. ;

Zerbini, M. ;

Zhou, X. ;

Fauquet, C. M. .

ARCHIVES OF VIROLOGY, 2008, 153 (04) :763-781

[2] Universal primers for the PCR-mediated amplification of DNA β -: A molecule associated with some monopartite begomoviruses [J].

Briddon, RW ;

Bull, SE ;

Mansoor, S ;

Amin, I ;

Markham, PG .

MOLECULAR BIOTECHNOLOGY, 2002, 20 (03) :315-318

[3] Identification of DNA components required for induction of cotton leaf curl disease [J].

Briddon, RW ;

Mansoor, S ;

Bedford, ID ;

Pinner, MS ;

Saunders, K ;

Stanley, J ;

Zafar, Y ;

Malik, KA ;

Markham, PG .

VIROLOGY, 2001, 285 (02) :234-243

[4] RETRACTED: Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana (Retracted article. See vol. 34, pg. 2595, 2015) [J].

Brigneti, G ;

Voinnet, O ;

Li, WX ;

Ji, LH ;

Ding, SW ;

Baulcombe, DC .

EMBO JOURNAL, 1998, 17 (22) :6739-6746

[5] Cladosporium fulvum CfHNNI1 induces hypersensitive necrosis, defence gene expression and disease resistance in both host and nonhost plants [J].

Cai, Xin-Zhong ;

Zhou, Xin ;

Xu, You-Ping ;

Joosten, Matthieu H. A. J. ;

de Wit, Pierre J. G. M. .

PLANT MOLECULAR BIOLOGY, 2007, 64 (1-2) :89-101

[6] Viral RNA silencing suppressors inhibit the microRNA pathway at an intermediate step [J].

Chapman, EJ ;

Prokhnevsky, AI ;

Gopinath, K ;

Dolja, VV ;

Carrington, JC .

GENES & DEVELOPMENT, 2004, 18 (10) :1179-1186

[7] Viral virulence protein suppresses RNA silencing-mediated defense but upregulates the role of MicroRNA in host gene expression [J].

Chen, J ;

Li, WX ;

Xie, DX ;

Peng, JR ;

Ding, SW .

PLANT CELL, 2004, 16 (05) :1302-1313

[8] A begomovirus DNAβ-encoded protein binds DNA, functions as a suppressor of RNA silencing, and targets the cell nucleus [J].

Cui, XF ;

Li, GX ;

Wang, DW ;

Hu, DW ;

Zhou, XP .

JOURNAL OF VIROLOGY, 2005, 79 (16) :10764-10775

[9] A DNAβ associated with Tomato Yellow Leaf Curl China Virus is required for symptom induction [J].

Cui, XF ;

Tao, XR ;

Xie, Y ;

Fauquet, CM ;

Zhou, XP .

JOURNAL OF VIROLOGY, 2004, 78 (24) :13966-13974

[10] Revising the way we conceive and name viruses below the species level: A review of geminivirus taxonomy calls for new standardized isolate descriptors [J].

Fauquet, CM ;

Stanley, J .

ARCHIVES OF VIROLOGY, 2005, 150 (10) :2151-2179

← 1 2 3 4 5 →