Nanoparticle-functionalized nucleic acids: A strategy for amplified electrochemical detection of some single-base mismatches

被引：11

作者：

Ahangar, Laleh Enayati ^{[1
]}

Mehrgardi, Masoud A. ^{[1
]}

机构：

[1] Univ Isfahan, Dept Chem, Esfahan 8174673441, Iran

来源：

ELECTROCHIMICA ACTA | 2011年 / 56卷 / 06期

关键词：

Single-base mismatches; DNA hybridization biosensor; Nanoparticle-functionalized nucleic acid; Thermodynamically stable mismatches; RANGE ELECTRON-TRANSFER; DNA HYBRIDIZATION BIOSENSOR; GLASSY-CARBON ELECTRODE; PROBE DNA; GRAPHITE; MERCURY; LABEL; ELECTROCATALYSIS; GUANINE; FILMS;

D O I：

10.1016/j.electacta.2010.12.051

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

In this study, nanoparticle-functionalized nucleic acids were employed to improve the sensitivity of electrochemical DNA biosensors that make capable them to detect different types of single-base mismatches (SBMs), including thermodynamically stable ones. The present biosensor was constructed by the immobilization of platinum nanoparticles (Pt-NPs) on the surface of a carbon paste electrode (CPE) via SH-functionalized DNA. A redox probe of 2-mercapto-1-methyl imidazole (MMI), which has different electrochemical behavior on Pt-NP and CPE, was used. This behavior helps to overcome the pinhole effect in DNA hybridization biosensors. Additionally, in the present biosensor, the positioning of the redox probe under the SBM in DNA, which decreases the sensitivity of most DNA biosensors, did not contribute to the observed electrochemical signal. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：2725 / 2729

页数：5

共 45 条

[11] Electrochemical detection of short sequences related to the hepatitis B virus using MB on chitosan-modified CPE
Guo Mandong
Li Yanqing
Guo Hongxia
Wu Xiaoqin
Fan Lifang
[J]. BIOELECTROCHEMISTRY, 2007, 70 (02) : 245 - 249
[12] Applications of carbon nanotubes in electrochemical DNA biosensors
He, PA
Xu, Y
Fang, YZ
[J]. MICROCHIMICA ACTA, 2006, 152 (3-4) : 175 - 186
[13] Electrochemical DNA Biosensor Based on Nanoporous Gold Electrode and Multifunctional Encoded DNA-Au Bio Bar Codes
Hu, Kongcheng
Lan, Dongxiao
Li, Xuemei
Zhang, Shusheng
[J]. ANALYTICAL CHEMISTRY, 2008, 80 (23) : 9124 - 9130
[14] Electroanalytical study of SYBR Green I and ethidium bromide intercalation in methylated and unmethylated amplicons
Ioannou, Andrea K.
Alexiadou, Despina K.
Kouidou, Sofia A.
Voulgaropoulos, Anastasios N.
Girousi, Stella Th.
[J]. ANALYTICA CHIMICA ACTA, 2010, 657 (02) : 163 - 168
[15] Electrochemical detection of enzyme labeled DNA based on disposable pencil graphite electrode
Kara, P
Erdem, A
Girousi, S
Ozsoz, M
[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2005, 38 (01) : 191 - 195
[16] Disposable electrochemical biosensor for the detection of the interaction between DNA and lycorine based on guanine and adenine signals
Karadeniz, H
Gulmez, B
Sahinci, F
Erdem, A
Kaya, GI
Unver, N
Kivcak, B
Ozsoz, M
[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2003, 33 (02) : 295 - 302
[17] Electrochemistry of methylene blue bound to a DNA-modified electrode
Kelley, SO
Barton, JK
Jackson, NM
Hill, MG
[J]. BIOCONJUGATE CHEMISTRY, 1997, 8 (01) : 31 - 37
[18] Kelley SO, 1999, ANGEW CHEM INT EDIT, V38, P941, DOI 10.1002/(SICI)1521-3773(19990401)38:7<941::AID-ANIE941>3.0.CO
[19] 2-7
[20] Kun W., 2009, ELECTROANAL, V21, P1159

← 1 2 3 4 5 →