Nanoparticle-functionalized nucleic acids: A strategy for amplified electrochemical detection of some single-base mismatches

被引：11

作者：

Ahangar, Laleh Enayati ^{[1
]}

Mehrgardi, Masoud A. ^{[1
]}

机构：

[1] Univ Isfahan, Dept Chem, Esfahan 8174673441, Iran

来源：

ELECTROCHIMICA ACTA | 2011年 / 56卷 / 06期

关键词：

Single-base mismatches; DNA hybridization biosensor; Nanoparticle-functionalized nucleic acid; Thermodynamically stable mismatches; RANGE ELECTRON-TRANSFER; DNA HYBRIDIZATION BIOSENSOR; GLASSY-CARBON ELECTRODE; PROBE DNA; GRAPHITE; MERCURY; LABEL; ELECTROCATALYSIS; GUANINE; FILMS;

D O I：

10.1016/j.electacta.2010.12.051

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

In this study, nanoparticle-functionalized nucleic acids were employed to improve the sensitivity of electrochemical DNA biosensors that make capable them to detect different types of single-base mismatches (SBMs), including thermodynamically stable ones. The present biosensor was constructed by the immobilization of platinum nanoparticles (Pt-NPs) on the surface of a carbon paste electrode (CPE) via SH-functionalized DNA. A redox probe of 2-mercapto-1-methyl imidazole (MMI), which has different electrochemical behavior on Pt-NP and CPE, was used. This behavior helps to overcome the pinhole effect in DNA hybridization biosensors. Additionally, in the present biosensor, the positioning of the redox probe under the SBM in DNA, which decreases the sensitivity of most DNA biosensors, did not contribute to the observed electrochemical signal. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：2725 / 2729

页数：5

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