A Nonenzymatic Amperometric Method for Fructosyl-Valine Sensing Using Ferroceneboronic Acid

Fructosyl valine (Fru-Val) is a glycosylated component of hemoglobin (HbA(1c)) that can serve as a diagnostic target for type 2 diabetes. While average blood glucose levels fluctuate significantly, the more stable levels of HbA1c can serve as a better long-term diagnostic marker. Here a diagnostic system, incorporating an amperometric method, for detecting Fru-Val (at + 0.1 V vs. Ag/AgCl), using ferrocene boronic acid (FcBA) is presented. FcBA can complex diols, and has easily detectable redox properties. The boronic acid group in FcBA mediates complexation, while the Fe(II)/Fe(III) couple serves as a transducer. The diagnostic system, based on a miniaturized bare glassy carbon paste electrode (GCPE), has a fast response time.