Swine acute diarrhea syndrome coronavirus (SADS-CoV) antagonizes interferon-β production via blocking IPS-1 and RIG-I

被引：28

作者：

Zhou, Zhihai ^{[1
,2
]}

Sun, Yuan ^{[1
,2
]}

Yan, Xiaoling ^{[1
,2
]}

Tang, Xiaoyu ^{[1
,2
]}

Li, Qianniu ^{[1
,2
]}

Tan, Yaorong ^{[1
,2
]}

Lan, Tian ^{[1
,2
]}

Ma, Jingyun ^{[1
,2
]}

机构：

[1] South China Agr Univ, Coll Anim Sci, Guangzhou, Peoples R China

[2] Key Lab Anim Hlth Aquaculture & Environm Control, Guangzhou, Guangdong, Peoples R China

来源：

VIRUS RESEARCH | 2020年 / 278卷

关键词：

Swine acute diarrhea syndrome coronavirus; IPS-1; RIG-I; Interferon beta; NF-KAPPA-B; ENTERIC ALPHACORONAVIRUS; VIRUS; INHIBITION; RECOGNITION; INDUCTION; ACTIVATION; INFECTION; CHINA; IFN;

D O I：

10.1016/j.virusres.2019.197843

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Swine acute diarrhea syndrome coronavirus (SADS-CoV), a newly emerging enteric coronavirus, is considered to be associated with swine acute diarrhea syndrome (SADS) which has caused significantly economic losses to the porcine industry. Interactions between SADS-CoV and the host innate immune response is unclear yet. In this study, we used IPEC-J2 cells as a model to explore potential evasion strategies employed by SADS-CoV. Our results showed that SADS-CoV infection failed to induce IFN-beta production, and inhibited poly (I:C) and Sendai virus (SeV)-triggered IFN-beta expression. SADS-CoV also blocked poly (I:C)-induced phosphorylation and nuclear translocation of IRF-3 and NF-kappa B. Furthermore, SADS-CoV did not interfere with the activity of IFN-beta promoter stimulated by IRF3, TBK1 and IKK epsilon, but counteracted its activation induced by IPS-1 and RIG-I. Collectively, this study is the first investigation that shows interactions between SADS-CoV and the host innate immunity, which provides information of the molecular mechanisms underlying SASD-CoV infection.

引用

页数：8

共 29 条

[1] The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-β promoter [J].