Evidence for multiple group 1 late embryogenesis abundant proteins in encysted embryos of Artemia and their organelles

被引:42
作者
Warner, Alden H. [1 ]
Miroshnychenko, Olga [1 ]
Kozarova, Anna [2 ]
Vacratsis, Panayiotis O. [2 ]
MacRae, Thomas H. [3 ]
Kim, Jinnie [3 ]
Clegg, James S. [4 ]
机构
[1] Univ Windsor, Dept Biol Sci, Windsor, ON N9B 3P4, Canada
[2] Univ Windsor, Dept Chem & Biochem, Windsor, ON N9B 3P4, Canada
[3] Dalhousie Univ, Dept Biol, Halifax, NS, Canada
[4] Univ Calif Davis, Sect Mol & Cellular Biol, Davis, CA 94923 USA
基金
加拿大自然科学与工程研究理事会;
关键词
artemia; desiccation tolerance; diapause; late embryogenesis abundant proteins; LEA proteins; SHOCK/ALPHA-CRYSTALLIN PROTEIN; RIBONUCLEIC-ACID POPULATIONS; DIAPAUSE-DESTINED EMBRYOS; HEAT-SHOCK-PROTEIN; BRINE SHRIMP; LEA PROTEINS; DEVELOPMENTAL BIOCHEMISTRY; COTTONSEED EMBRYOGENESIS; DESICCATION TOLERANCE; STRESS TOLERANCE;
D O I
10.1093/jb/mvq091
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The presence of late embryogenesis abundant (LEA) proteins in plants and animals has been linked to their ability to tolerate a variety of environmental stresses. Among animals, encysted embryos of the brine shrimp Artemia franciscana are among the most stress resistant eukaryotes, and for that reason it is considered to be an extremophile. The study presented here demonstrates that these embryos contain multiple group 1 LEA proteins with masses of 21, 19, 15.5 and 13 kDa. The LEA proteins first appear in diapause-destined embryos, beginning at similar to 4 days post-fertilization, but not in nauplii-destined embryos. After resumption of embryonic development, the LEA proteins decline slowly in the desiccation resistant encysted stages, then disappear rapidly as the embryo emerges from its shell. LEA proteins are absent in fully emerged embryos, larvae and adults. They are abundant in mitochondria of encysted embryos, but barely detectable in nuclei and absent from yolk platelets. LEA proteins were also detected in dormant embryos of six other species of Artemia from hypersaline environments around the world. This study enhances our knowledge of the group 1 LEA proteins in stress tolerant crustacean embryos.
引用
收藏
页码:581 / 592
页数:12
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