Melatonin: quantitative analysis in pharmaceutical oral dosage forms using thin-layer chromatography (TLC) densitometry

被引：12

作者：

Costantini, A ^{[1
]}

Paoli, F ^{[1
]}

机构：

[1] Stabilimento Chim Farmaceut Militare, I-50141 Florence, Italy

来源：

FARMACO | 1998年 / 53卷 / 06期

关键词：

melatonin; densitometry; thin-layer chromatography; oral dosage forms;

D O I：

10.1016/S0014-827X(98)00040-8

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A thin-layer chromatography densitometric method for the quantitative analysis of melatonin in tablets and capsules has been developed. High-performance thin-layer chromatography (HPTLC) silica gel plates were used as the stationary phase and the elution was made with CH2Cl2/MeOH (95:5). Densitometric evaluation of the spots was performed with a TLC scanner in reflectance mode at 223 nm with a deuterium lamp. A linear relation between the peak areas and the amount of melatonin deposited was found in the range 63-399 ng. The method was applied with success for the quantitative determination of melatonin in commercial and experimental samples. (C) 1998 Elsevier Science S.A. All rights reserved.

引用

页码：443 / 447

页数：5

共 54 条

[21] Melatonin determination in human urine by liquid chromatography using flurometric detection [J].

Hariharan, M ;

Naickar, JS ;

Hilibrand, AS .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1996, 19 (11) :1745-1756

[22] Simultaneous determination of serotonin, N-acetylserotonin and melatonin in the pineal gland of the juvenile golden hamster by high-performance liquid chromatography with electrochemical detection [J].

Harumi, T ;

Akutsu, H ;

Matsushima, S .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 675 (01) :152-156

[23] Hydroxyindole-O-methyltransferase activity assay using high-performance liquid chromatography with fluorometric detection: Determination of melatonin enzymatically formed from N-acetylserotonin and S-adenosyl-L-methionine [J].

Itoh, MT ;

Hattori, A ;

Sumi, Y .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1997, 692 (01) :217-221

[24]

JAN JE, 1994, DEV MED CHILD NEUROL, V36, P97

[25] Use of melatonin in the treatment of paediatric sleep disorders [J].

Jan, JE ;

ODonnell, ME .

JOURNAL OF PINEAL RESEARCH, 1996, 21 (04) :193-199

[26] Melatonin affords protection against gastric lesions induced by ischemia-reperfusion possibly due to its antioxidant and mucosal microcirculatory effects [J].

Konturek, PC ;

Konturek, SJ ;

Majka, J ;

Zembala, M ;

Hahn, EG .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1997, 322 (01) :73-77

[27] SENSITIVE ASSAY FOR MELATONIN IN HUMAN SERUM BY LIQUID-CHROMATOGRAPHY [J].

LAGANA, A ;

MARINO, A ;

FAGO, G ;

PARDOMARTINEZ, B ;

BIZZARRI, M .

ANALYTICA CHIMICA ACTA, 1995, 316 (03) :377-385

[28] MELATONIN-LIKE IMMUNOREACTIVITY IN THE PRESENCE OF DIFFERENT CHEMICALS AS DETERMINED BY THE RADIOIMMUNOASSAY [J].

LAHIRI, DK ;

DAVIS, D ;

NURNBERGER, JI .

BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY, 1994, 51 (01) :51-54

[29] FACTORS THAT INFLUENCE RADIOIMMUNOASSAY OF HUMAN PLASMA MELATONIN - A MODIFIED COLUMN PROCEDURE TO ELIMINATE INTERFERENCE [J].

LAHIRI, DK ;

DAVIS, D ;

ADKINS, M ;

NURNBERGER, JI .

BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY, 1993, 49 (01) :36-50

[30] DETERMINATION OF MELATONIN BY GC-MS - PROBLEMS WITH SOLID-PHASE EXTRACTION (SPE) COLUMNS [J].

LEE, CR ;

ESNAUD, H .

BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1988, 15 (12) :677-679

← 1 2 3 4 5 6 →