Assessment of genetic diversity in cowpea (Vigna unguiculata L. Walp.) through ISSR marker

被引：0
|
作者：
Saxena, A. [1 ]
Rukam, Tomar S. [1 ]
机构：
[1] Junagadh Agr Univ, Dept Biotechnol, Junagadh, Gujarat, India
来源：
RESEARCH JOURNAL OF BIOTECHNOLOGY | 2020年 / 15卷 / 03期
关键词：
Cowpea; Morphological; ISSR; AMOVA; PCoA; SEQUENCE REPEAT DNA; POLYMORPHISMS;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Cowpea is a self-pollinated lagumenous crop showing different growth habits and morphological traits, but their genetic and geographical variations are not clear. Present study was therefore planned to find diversity based on morphological and molecular markers in fifty-six cowpea genotypes originated from different regions. Analysis using twenty polymorphic ISSR markers, an average 88.93% polymorphism was found with polymorphic information content and resolving power of 0.783 and 2.74 respectively. Analysis of molecular variance exhibited that different genotypes and markers under study have significant variance at genetic level. Dendrogram grouping indicated Jaccard similarity range of 52.00 to 83.00 % mostly based upon their place of origin and morphological characters while principal coordinate analysis exhibited clustering based upon growth habits. Present diversity analysis revealed that cowpea genotypes can be differentiated using ISSR markers. Genotypes based upon genetic, morphological and geographical differences may be chosen for developing a breeding programme.
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页码：66 / 71
页数：6
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