Oxyresveratrol stimulates mucin production in an NAD+-dependent manner in human intestinal goblet cells

被引:20
作者
Hwang, Dahyun [1 ]
Jo, HyunA. [2 ]
Ma, Seong-Ho [2 ]
Lim, Young-Hee [2 ,3 ,4 ]
机构
[1] Hoseo Univ, Coll Life & Hlth Sci, Dept Biomed Lab Sci, Asan 31499, South Korea
[2] Korea Univ, Grad Sch, Dept Integrated Biomed & Life Sci, Seoul 02841, South Korea
[3] Korea Univ, Grad Sch, PLUS Program BK21, Dept Publ Hlth Sci, Seoul 02841, South Korea
[4] Korea Univ, Guro Hosp, Dept Lab Med, Seoul 08308, South Korea
基金
新加坡国家研究基金会;
关键词
Oxyresveratrol; NAD(+); MUC2; Goblet cells; Intestinal mucus layer; NICOTINAMIDE MONONUCLEOTIDE; NAD(+) LEVELS; RESVERATROL; CANCER; MICE; COMMUNICATION; PROTECTION; COLITIS; ACID; DIET;
D O I
10.1016/j.fct.2018.06.039
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The intestinal mucus layer plays an important role in the management of inflammatory bowel disease. The aim of this study was to investigate the effects of oxyresveratrol (OXY), an antioxidant, on the stimulation of mucin production in human LS 174T goblet cells and the underlying mechanism thereof. OXY increased MUC2 expression at both the mRNA and protein levels. By performing two-dimensional gel electrophoresis, we found that the expression of nicotinic acid phosphoribosyltransferasel (NaPRT1) in OXY-treated LS 174T cells was greatly increased compared with that in negative control cells. In addition, the NAD(+)/NADH ratio was increased in proportion to OXY in LS 174T cells. The expression of NAD(+)-synthesis enzymes, NaPRT1, nicotinamide riboside kinase1 (NRK1) and nicotinamide mononucleotide adenylyltransferasel (Nmnat1) was significantly increased at both the mRNA and protein levels in OXY-treated LS 174T cells. The inhibition of NaPRT1 and NRK1 did not decrease MUC2 expression after inhibiting by small interfering RNA (siRNA)-NaPRT1 and siRNA-NRK1, respectively; however, inhibition of Nmnat by an Nmnat inhibitor decreased MUC2 expression in a dose-dependent manner. In conclusion, OXY increases NAD(+) levels, resulting in the stimulation of MUC2 expression in LS 174T cells. These findings present a novel role for NAD(+) in stimulation of MUC2 expression.
引用
收藏
页码:880 / 888
页数:9
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