Polystyrene nanoparticles induced neurodevelopmental toxicity in Caenorhabditis elegans through regulation of dpy-5 and rol-6

被引：39

作者：

Shang, Yu ^{[1
]}

Wang, Siyan ^{[1
]}

Jin, Yingying ^{[1
]}

Xue, Wanlei ^{[1
]}

Zhong, Yufang ^{[1
]}

Wang, Hongli ^{[2
]}

An, Jing ^{[1
]}

Li, Hui ^{[1
]}

机构：

[1] Shanghai Univ, Sch Environm & Chem Engn, Nanchen Rd 333, Shanghai 200444, Peoples R China

[2] Shanghai Acad Environm Sci, State Environm Protect Key Lab Format & Prevent U, Shanghai 200233, Peoples R China

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2021年 / 222卷

基金：

中国国家自然科学基金;

关键词：

Polystyrene nanoparticles; Caenorhabditis elegans; Neurodevelopmental toxicity; Oxidative damage; MICROPLASTICS; COLLAGEN; TISSUE; DAMAGE; GENE; SIZE;

D O I：

10.1016/j.ecoenv.2021.112523

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Micro- and nano- polystyrene particles have been widely detected in environment, posing potential threats to human health. This study was designed to evaluate the neurodevelopmental toxicity of polystyrene nanoparticles (NPs) in Caenorhabditis elegans (C. elegans), to screen crucial genes and investigate the underlying mechanism. In wild-type C. elegans, polystyrene NPs (diameter 50 nm) could concentration-dependently induce significant inhibition in body length, survival rate, head thrashes, and body bending, accompanying with increase of reactive oxygen species (ROS) production, lipofuscin accumulation, and apoptosis and decrease of dopamine (DA) contents. Moreover, pink-1 mutant was demonstrated to alleviate the locomotion disorders and oxidative damage induced by polystyrene NPs, indicating involvement of pink-1 in the polystyrene NPs-induced neurotoxicity. RNA sequencing results revealed 89 up-regulated and 56 down-regulated differently expressed genes (DEGs) response to polystyrene NPs (100 mu g/L) exposure. Gene Ontology (GO) enrichment analysis revealed that predominant enriched DEGs were correlated with biological function of cuticle development and molting cycle. Furthermore, mutant strains test showed that the neurodevelopmental toxicity and oxidative stress responses induced by 50 nm polystyrene NPs were regulated by dpy-5 and rol-6. In general, polystyrene NPs induced obvious neurodevelopmental toxicity in C. elegans through oxidative damage and dopamine reduction. Crucial genes dpy-5 and rol-6 might participate in polystyrene NPs-induced neurodevelopmental toxicity through regulation on synthesis and deposition of cuticle collagen.

引用

页数：9

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