Multi-drug resistant Escherichia coli in diarrhoeagenic foals: Pulsotyping, phylotyping, serotyping, antibiotic resistance and virulence profiling

被引:17
|
作者
Kennedy, C. A. [1 ]
Walsh, C. [2 ,3 ]
Karczmarczyk, M. [3 ]
O'Brien, S. [3 ]
Akasheh, N. [4 ]
Quirke, M. [2 ]
Farrell-Ward, S. [3 ]
Buckley, T. [5 ]
Fogherty, U. [5 ]
Kavanagh, K. [5 ]
Parker, C. T. [6 ]
Sweeney, T.
Fanning, S. [3 ]
机构
[1] Univ Coll Dublin, UCD Vet Sci Ctr, Dublin 4, Ireland
[2] DIT, Sch Food Sci & Environm Hlth, Cathal Brugha St, Dublin D01 HV58, Ireland
[3] Univ Coll Dublin, Sch Publ Hlth Physiotherapy & Sports Sci, UCD Ctr Food Safety, Dublin D04 N2E5, Ireland
[4] St James Hosp, Med Directorate, Dublin 8, Ireland
[5] Irish Equine Ctr, Naas W91 RH93, Kildare, Ireland
[6] ARS, Prod Safety & Microbiol Res Unit, USDA, 800 Buchanan St, Albany, CA 94710 USA
关键词
Escherichia coli; Diarrhoeagenic; Foals; Serotyping; Antibiotic resistance; Adherence and invasion PFGE; ExPEC; ESBL; FLUOROQUINOLONE RESISTANCE; MOLECULAR CHARACTERIZATION; STRAINS; GENE; ANIMALS; HORSES; IMPACT; FECES; FOOD; EPIDEMIOLOGY;
D O I
10.1016/j.vetmic.2018.08.009
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Extraintestinal pathogenic E. coli (ExPEC) possess the ability to cause extraintestinal infections such as urinary tract infections, neonatal meningitis and sepsis. While information is readily available describing pathogenic E. con populations in food-producing animals, studies in companion/sports animals such as horses are limited. In addition, many antimicrobial agents used in the treatment of equine infections are also utilised in human medicine, potentially contributing to the spread of antibiotic resistance determinants among pathogenic strains. The aim of this study was to phenotypically and genotypically characterise the multidrug resistance and virulence associated with 83 equine E. con isolates recovered from foals with diarrhoeal disease. Serotyping was performed by both PCR and sequencing. Antibiotic resistance was assessed by disc diffusion. Phylogenetic groups, virulence genes, antibiotic resistance genes and integrons were determined by PCR. Thirty-nine (46%) of the isolates were classified as ExPEC and hence considered to be potentially pathogenic to humans and animals. Identified serogroups 01, 019a, 040, 0101 and 0153 are among previously reported human clinical ExPEC isolates. Over a quarter of the E. call were assigned to pathogenic phylogroups B2 (6%) and D (23%). Class 1 and class 2 integrons were detected in 85% of E. coil, revealing their potential to transfer MDR to other pathogenic and non-pathogenic bacteria. With 65% of potentially pathogenic isolates harbouring one or more TEM, SHV and CTX-M-2 group beta-lactamases, in addition to the high levels of resistance to fluoroquinolones observed, our findings signal the need for increased attention to companion/sport animal reservoirs as public health threats.
引用
收藏
页码:144 / 152
页数:9
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