High performance CCD camera system for digitalisation of 2D DIGE gels

被引：7

作者：

Strijkstra, Annemieke ^{[1
,2
]}

Trautwein, Kathleen ^{[1
,2
]}

Roesler, Stefan ^{[3
]}

Feenders, Christoph ^{[4
]}

Danzer, Daniel ^{[3
]}

Riemenschneider, Udo ^{[3
]}

Blasius, Bernd ^{[4
]}

Rabus, Ralf ^{[1
,2
]}

机构：

[1] Carl von Ossietzky Univ Oldenburg, Gen & Mol Microbiol, Inst Chem & Biol Marine Environm ICBM, Carl von Ossietzky Str 9-11, D-26111 Oldenburg, Germany

[2] Max Planck Inst Marine Mikrobiol, Dept Microbiol, Bremen, Germany

[3] Intas Sci Imaging Instruments GmbH, Gottingen, Germany

[4] Carl von Ossietzky Univ Oldenburg, Math Modelling, Inst Chem & Biol Marine Environm ICBM, Oldenburg, Germany

来源：

PROTEOMICS | 2016年 / 16卷 / 14期

关键词：

2D DIGE; CCD camera; Laser scanner; Intas Advanced 2D Imager; Technology; Typhoon; MARINE;

D O I：

10.1002/pmic.201500385

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An essential step in 2D DIGE-based analysis of differential proteome profiles is the accurate and sensitive digitalisation of 2D DIGE gels. The performance progress of commercially available charge-coupled device (CCD) camera-based systems combined with light emitting diodes (LED) opens up a new possibility for this type of digitalisation. Here, we assessed the performance of a CCD camera system (Intas Advanced 2D Imager) as alternative to a traditionally employed, high-end laser scanner system (Typhoon 9400) for digitalisation of differential protein profiles from three different environmental bacteria. Overall, the performance of the CCD camera system was comparable to the laser scanner, as evident from very similar protein abundance changes (irrespective of spot position and volume), as well as from linear range and limit of detection.

引用

页码：1975 / 1979

页数：5

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