Bazi Bushen Capsule Alleviates Post-Menopausal Atherosclerosis via GPER1-Dependent Anti-Inflammatory and Anti-Apoptotic Effects

被引：25
作者：
Huang, Dan [1 ,2 ]
Wang, Xindong [1 ,2 ]
Zhu, Yunhong [1 ,2 ]
Gong, Juexiao [1 ,2 ]
Liang, Junqing [3 ]
Song, Yanfei [3 ]
Zhang, Yiyan [1 ,2 ]
Liu, Linsheng [4 ]
Wei, Cong [3 ]
机构：
[1] Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese Western, Nanjing, Peoples R China
[2] Jiangsu Prov Acad Tradit Chinese Med, Nanjing, Peoples R China
[3] Natl Key Lab Collateral Dis Res & Innovat Chinese, Shijiazhuang, Hebei, Peoples R China
[4] Soochow Univ, Dept Clin Pharmacol, Affiliated Hosp 1, Suzhou, Peoples R China
来源：
FRONTIERS IN PHARMACOLOGY | 2021年 / 12卷
基金：
中国国家自然科学基金;
关键词：
atherosclerosis; bazi bushen capsule; lipid profile; inflammation; apoptosis; GPER1; antagonist; NF-KAPPA-B; ESTROGEN-RECEPTOR; ENDOTHELIAL-CELLS; PREVENT ATHEROSCLEROSIS; LIPID-METABOLISM; INFLAMMATION; ACTIVATION; ALPHA; GPER;
D O I：
10.3389/fphar.2021.658998
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Bazi Bushen capsule (BZBS), as a Chinese medicine used to relieve fatigue, has been proven effective for the treatment of atherogenesis through antilipid effects. To investigate the potential mechanism of BZBS in the anti-atherosclerotic effect, Ovx/ApoE(-/-) mice were applied to investigate the anti-atherosclerotic efficiency and potential mechanism of BZBS. Therapeutic effect was evaluated based on the number of CD68(+) and CD3(+) cells, the level of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), and the ratio of cleaved caspase-3/caspase-3, as well as increasing ratio of Bcl2/Bax. Human umbilical vein endothelial cells (HUVECs) were chosen to evaluate the role of GPER1. Treatment with BZBS reduced lipid deposition by reducing the numbers of CD68(+) and CD3(+) cells, the level of ICAM-1 and VCAM-1, and the ratio of cleaved caspase-3/caspase-3, and increasing the ratio of Bcl2/Bax as compared with the control group. In si-GPER1-treated HUVECs, the anti-apoptotic effect of BZBS was decreased. This study revealed that BZBS exhibited a clear effect against atherogenesis via GPER1-dependent anti-inflammatory and anti-apoptotic mechanisms. We believe that this manuscript is informative and useful for researchers pursuing the related alleviation of post-menopausal AS via anti-inflammatory and anti-apoptotic mechanisms.
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页数：12
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