Limited field radiation therapy results in decreased bone fracture toughness in a murine model

被引:22
作者
Bartlow, Christopher M. [1 ]
Mann, Kenneth A. [1 ]
Damron, Timothy A. [1 ]
Oest, Megan E. [1 ]
机构
[1] SUNY Upstate Med Univ, Dept Orthoped Surg, Syracuse, NY 13210 USA
来源
PLOS ONE | 2018年 / 13卷 / 10期
基金
美国国家卫生研究院;
关键词
HUMAN CORTICAL BONE; GLYCATION END-PRODUCTS; COLLAGEN CROSS-LINKS; SOFT-TISSUE SARCOMA; AGE-RELATED-CHANGES; X-RAY-IRRADIATION; BIOMECHANICAL PROPERTIES; NONENZYMATIC GLYCATION; IONIZING-RADIATION; GAMMA-IRRADIATION;
D O I
10.1371/journal.pone.0204928
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Fragility fractures are a well-known complication following oncologic radiotherapy, and it is suspected that radiation-induced embrittlement of bone within the treatment field may contribute to fracture risk. To explore this phenomenon, a mouse model (BALB/cJ) of fractionated, limited field, bilateral hindlimb irradiation (4x5 Gy) was used. The effects of radiation on femoral (cortical) bone fracture toughness, morphology, and biochemistry-including advanced glycation end products (AGEs)-were quantified and compared to Sham group samples prior to irradiation and at 0, 4, 8, and 12 weeks post-irradiation. Additionally, alterations to bone fracture toughness mediated directly by radiation (independent of cellular mechanisms) were determined using devitalized mouse cadaver femurs. Finally, the contribution of AGEs to reduced fracture toughness was examined by artificially ribosylating mouse femurs ex vivo. These data demonstrate that in vivo irradiation results in an immediate (-42% at 0 weeks, p < 0.001) and sustained (-28% at 12 weeks, p < 0.001) decrease in fracture toughness with small changes in morphology (-5% in cortical area at 12 weeks), and minimal changes in bone composition (tissue mineral density, mineral:matrix ratio, and AGE content). Irradiation of devitalized femurs also reduced fracture toughness (-29%, p < 0.001), but to a lesser extent than was seen in vivo. While artificial ribosylation decreased fracture toughness with time, the extent of glycation needed to induce this effect exceeded the AGE accumulation that occurred in vivo. Overall, hindlimb irradiation induced a substantial and sustained decrease in bone fracture toughness. Approximately half of this decrease in fracture toughness is due to direct radiation damage, independent of cellular remodeling. Collagen glycation in vivo was not substantially altered, suggesting other matrix changes may contribute to post-radiotherapy bone embrittlement.
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页数:22
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