Vaccination of chickens with DNA vaccine expressing Eimeria tenella MZ5-7 against coccidiosis

被引：32
作者：
Geriletu [1 ]
Xu, Lixin [1 ]
Xurihua [2 ]
Li, Xiangrui [1 ]
机构：
[1] Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China
[2] Inner Mongolia Agr Univ, Coll Vet Med, Hohhot 010018, Inner Mongolia, Peoples R China
来源：
VETERINARY PARASITOLOGY | 2011年 / 177卷 / 1-2期
关键词：
Eimeria tenella; MZ5-7; DNA vaccine; IL17; Chicken; PROTECTIVE IMMUNITY; INTERFERON-GAMMA; INTERLEUKIN-17; FAMILY; RESPONSES; ANTIGEN; IMMUNIZATION; STRATEGIES; INFECTION; CYTOKINES; ANTIBODY;
D O I：
10.1016/j.vetpar.2010.11.041
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A chimeric DNA vaccine co-expressing Eimeria tenella MZ5-7 and chicken IL-17 gene was constructed and its efficacy against E. tenella challenge was evaluated. The ORF of MZ5-7 from E. tenella's second generation merozoite and the mature interleukin 17 gene of chicken were cloned into the expression vector of pcDNA4.0 to construct DNA vaccine pcDNA4.0-MZ and pcDNA4.0-MZ-IL17. The expression of aim gene products in vivo was detected by western blotting. The expression of IL-2 and IFN-gamma in chicken splenocytes was detected by RT-PCR 7 days post-immunization. The expression levels of the two cytokines in the pcDNA4.0-MZ-IL17 DNA vaccine immunized group were significantly higher than that in the pcDNA4.0-MZ immunized group (p < 0.05). Either pcDNA4.0-MZ or pcDNA4.0-MZ-IL17 DNA vaccine could obviously alleviate cecal lesions, body weight loss and increase oocyst decrease ratio. The ACI of pcDNA4.0-MZ-IL17 group was 190, which is higher than that of pcDNA4.0-MZ group and all the three control groups. In short, MZ5-7 was an effective candidate antigen for vaccine and co-expression of cytokine with antigen was an alternative method to enhance the immunity DNA vaccine. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码：6 / 12
页数：7
相关论文
共 32 条
[1]   Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry [J].
Allen, PC ;
Fetterer, RH .
CLINICAL MICROBIOLOGY REVIEWS, 2002, 15 (01) :58-+
[2]   CLONING AND CHARACTERIZATION OF A SURFACE-ANTIGEN OF EIMERIA-TENELLA MEROZOITES AND EXPRESSION USING A RECOMBINANT VACCINIA VIRUS [J].
BINGER, MH ;
HUG, D ;
WEBER, G ;
SCHILDKNECHT, E ;
HUMBELIN, M ;
PASAMONTES, L .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1993, 61 (02) :179-187
[3]   Antibody response against endogenous stages of an attenuated strain of Eimeria tenella [J].
Constantinoiu, C. C. ;
Molloy, J. B. ;
Jorgensen, W. K. ;
Coleman, G. T. .
VETERINARY PARASITOLOGY, 2008, 154 (3-4) :193-204
[4]   Recent advances in immunomodulation and vaccination strategies against coccidiosis [J].
Dalloul, RA ;
Lillehoj, HS .
AVIAN DISEASES, 2005, 49 (01) :1-8
[5]   Protective immunity against Eimeria acervulina following in ovo immunization with a recombinant subunit vaccine and cytokine genes [J].
Ding, XC ;
Lillehoj, HS ;
Quiroz, MA ;
Bevensee, E ;
Lillehoj, EP .
INFECTION AND IMMUNITY, 2004, 72 (12) :6939-6944
[6]  
Huang SH, 2004, ALLERGY ASTHMA PROC, V25, P17
[7]   Regulation of mucosal IgA responses in vivo: Cytokines and adjuvants [J].
Husband, AJ ;
Kramer, DR ;
Bao, S ;
Sutherland, RM ;
Beagley, KW .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1996, 54 (1-4) :179-186
[8]   ANTICOCCIDIAL DRUGS - LESION SCORING TECHNIQUES IN BATTERY AND FLOOR-PEN EXPERIMENTS WITH CHICKENS [J].
JOHNSON, J ;
REID, WM .
EXPERIMENTAL PARASITOLOGY, 1970, 28 (01) :30-+
[9]   Modulation of antigen-specific cellular immune responses to DNA vaccination in rhesus macaques through the use of IL-2, IFN-γ, or IL-4 gene adjuvants [J].
Kim, JJ ;
Yang, JS ;
Manson, KH ;
Weiner, DB .
VACCINE, 2001, 19 (17-19) :2496-2505
[10]   Responses of chickens to a recombinant refractile body antigen of Eimeria tenella administered using various immunizing strategies [J].
Kopko, SH ;
Martin, DS ;
Barta, JR .
POULTRY SCIENCE, 2000, 79 (03) :336-342
1234