Detection of the glmM Gene in Helicobacter pylori Isolates with a Novel Primer by PCR
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Cordova Espinoza, Maria Guadalupe
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机构:IPN, Escuela Nacl Ciencias Biol, Lab Bacteriol Med, Dept Microbiol, Mexico City 11340, DF, Mexico
Cordova Espinoza, Maria Guadalupe
Gonzalez Vazquez, Rosa
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Ctr Med Nacl La Raza IMSS, Mexico City, DF, MexicoIPN, Escuela Nacl Ciencias Biol, Lab Bacteriol Med, Dept Microbiol, Mexico City 11340, DF, Mexico
Gonzalez Vazquez, Rosa
[2
]
Morales Mendez, Iyari
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INPer, Mexico City, DF, MexicoIPN, Escuela Nacl Ciencias Biol, Lab Bacteriol Med, Dept Microbiol, Mexico City 11340, DF, Mexico
Morales Mendez, Iyari
[3
]
Ruelas Vargas, Consuelo
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Ctr Med Nacl La Raza IMSS, Mexico City, DF, MexicoIPN, Escuela Nacl Ciencias Biol, Lab Bacteriol Med, Dept Microbiol, Mexico City 11340, DF, Mexico
Ruelas Vargas, Consuelo
[2
]
Giono Cerezo, Silvia
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IPN, Escuela Nacl Ciencias Biol, Lab Bacteriol Med, Dept Microbiol, Mexico City 11340, DF, MexicoIPN, Escuela Nacl Ciencias Biol, Lab Bacteriol Med, Dept Microbiol, Mexico City 11340, DF, Mexico
Giono Cerezo, Silvia
[1
]
机构:
[1] IPN, Escuela Nacl Ciencias Biol, Lab Bacteriol Med, Dept Microbiol, Mexico City 11340, DF, Mexico
[2] Ctr Med Nacl La Raza IMSS, Mexico City, DF, Mexico
A novel reverse primer (GLM MR1) was designed for detection of the glmM gene in Helicobacter pylori by PCR. The percentage of amplification in clinical isolates using GLM MR1 was 100% for detection of the glmM gene and 86.36% for the ureA gene. The primer designed is useful for the identification of H. pylori.