Reactive oxygen species in photochemistry of the red fluorescent protein "Killer Red"

被引：101

作者：

Vegh, Russell B. ^{[1
,2
]}

Solntsev, Kyril M. ^{[1
]}

Kuimova, Marina K. ^{[3
]}

Cho, Soohee ^{[1
,2
]}

Liang, Yue ^{[2
,4
]}

Loo, Bernard L. W. ^{[2
,4
]}

Tolbert, Laren M. ^{[1
]}

Bommarius, Andreas S. ^{[1
,2
,4
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

[2] Georgia Inst Technol, Parker H Petit Inst Bioengn & Biosci, Atlanta, GA 30332 USA

[3] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

[4] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 17期

基金：

美国国家科学基金会; 英国工程与自然科学研究理事会; 美国国家卫生研究院;

关键词：

SUPEROXIDE RADICAL ADDUCTS; STATE DYNAMICS; CHROMOPHORE; CELLS; HYDROXYL; DMPO; DNA;

D O I：

10.1039/c0cc05713d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The fluorescent protein aptly named "Killer Red" (KRed) is capable of killing transfected cells and inactivating fused proteins upon exposure to visible light in the presence of oxygen. We have investigated the source of the bioactive species through a variety of photophysical and photochemical techniques. Our results indicate a Type I (electron transfer mediated) photosensitizing mechanism.

引用

页码：4887 / 4889

页数：3

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