The role of SDF-1 in homing of human adipose-derived stem cells

被引：42
作者：
Stuermer, Ewa K. [1 ]
Lipenksy, Alexandra [2 ]
Thamm, Oliver [2 ]
Neugebauer, Edmund [1 ]
Schaefer, Nadine [1 ]
Fuchs, Paul [2 ]
Bouillon, Bertil [3 ]
Koenen, Paola [3 ]
机构：
[1] Univ Witten Herdecke, Inst Res Operat Med IFOM, D-51109 Cologne, Germany
[2] Univ Witten Herdecke, Handsurg Burn Care Ctr, Clin Plast & Reconstruct Surg, D-51109 Cologne, Germany
[3] Univ Witten Herdecke, Cologne Merheim Med Ctr, Dept Trauma & Orthoped Surg, D-51109 Cologne, Germany
来源：
WOUND REPAIR AND REGENERATION | 2015年 / 23卷 / 01期
关键词：
STROMAL CELLS; CHEMOKINE RECEPTOR; TISSUE INHIBITOR; BRAIN-TUMORS; FACTOR-I; EXPRESSION; MATRIX; SDF-1-ALPHA; MIGRATION; THERAPY;
D O I：
10.1111/wrr.12248
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
One of the putative pathophysiological mechanisms of chronic wounds is a disturbed homing of stem cells. In this project, the stromal cell-derived factor 1 (SDF-1)/C-X-C chemokine receptor (CXCR) 4 and SDF-1/CXCR7 pathway were focused in human adipose-derived stem cells (ASCs). ASCs were incubated with acute (AWF) or chronic wound fluid (CWF) to analyze their effects by quantitative real-time polymerase chain reaction (SDF-1, CXCR4, CXCR7, TIMP3), enzyme-linked immunosorbent assay (SDF-1 in WFs and supernatant), and transwell migration assay with/without antagonization. Whereas SDF-1 amounted 73.5 pg/mL in AWF, it could not be detected in CWF. Incubation with AWF led to a significant enhancement (129.7 pg/mL vs. 95.5 pg/mL), whereas CWF resulted in a significant reduction (30 pg/mL vs. 95.5 pg/mL) of SDF-1 in ASC supernatant. The SDF-1 receptor CXCR7 was detected on ASCs. AWF but not CWF significantly induced ASC migration, which was inhibited by CXCR4 and CXCR7 antagonists. Expressions of SDF-1, CXCR4, and CXCR7 were significantly stimulated by AWF while TIMP3 expression was reduced. In conclusion, an uncontrolled inflammation in the chronic wound environment, indicated by a reduced SDF-1 expression, resulted in a decreased ASC migration. A disturbed SDF-1/CXCR4 as well as SDF-1/CXCR7 pathway seems to play an important role in the impaired healing of chronic wounds.
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收藏
页码：82 / 89
页数：8
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