Heteromeric complexes of heat shock protein 70 (HSP70) family members, including Hsp70B', in differentiated human neuronal cells

被引：20

作者：

Chow, Ari M. ^{[1
]}

Mok, Philip ^{[1
]}

Xiao, Dawn ^{[1
]}

Khalouei, Sam ^{[1
]}

Brown, Ian R. ^{[1
]}

机构：

[1] Univ Toronto Scarborough, Ctr Neurobiol Stress, Toronto, ON M1C 1A4, Canada

来源：

CELL STRESS & CHAPERONES | 2010年 / 15卷 / 05期

关键词：

SH-SY5Y neuronal cells; Hsp70B '; Hsp70; Hsc70; Heteromeric complexes; Celastrol; HEAT-SHOCK PROTEINS; MOLECULAR CHAPERONE HSC70; HUMAN COLON CELLS; MESSENGER-RNA; PARKINSONS-DISEASE; RABBIT CEREBELLUM; PC12; CELLS; STRESS; EXPRESSION; INDUCTION;

D O I：

10.1007/s12192-009-0167-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Human neurodegenerative disorders such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis have been termed "protein misfolding disorders." Upregulation of heat shock proteins that target misfolded aggregation-prone proteins has been proposed as a potential therapeutic strategy to counter neurodegenerative disorders. The heat shock protein 70 (HSP70) family is well characterized for its cytoprotective effects against cell death and has been implicated in neuroprotection by overexpression studies. HSP70 family members exhibit sequence and structural conservation. The significance of the multiplicity of HSP70 proteins is unknown. In this study, coimmunoprecipitation was employed to determine if association of HSP70 family members occurs, including Hsp70B' which is present in the human genome but not in mouse and rat. Heteromeric complexes of Hsp70B', Hsp70, and Hsc70 were detected in differentiated human SH-SY5Y neuronal cells. Hsp70B' also formed complexes with Hsp40 suggesting a common co-chaperone for HSP70 family members.

引用

页码：545 / 553

页数：9

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