Changing Technologies of RNA Sequencing and Their Applications in Clinical Oncology

被引：82

作者：

Wang, Ye ^{[1
]}

Mashock, Michael ^{[2
]}

Tong, Zhuang ^{[3
]}

Mu, Xiaofeng ^{[1
,4
]}

Chen, Hong ^{[5
]}

Zhou, Xin ^{[5
]}

Zhang, Hong ^{[2
]}

Zhao, Gexin ^{[2
]}

Liu, Bin ^{[3
]}

Li, Xinmin ^{[2
]}

机构：

[1] Qingdao Univ, Qingdao Cent Hosp, Clin Lab, Affiliated Hosp 2,Med Coll, Qingdao, Peoples R China

[2] Univ Calif Los Angeles, Dept Pathol & Lab Med, Technol Ctr Genom & Bioinformat, Los Angeles, CA 90095 USA

[3] China Med Univ, Liaoning Canc Hosp & Inst, Canc Hosp, Shenyang, Peoples R China

[4] Tianjin Univ, Acad Med Engn & Translat Med, Tianjin, Peoples R China

[5] Qiqihaer First Hosp, Qiqihar, Peoples R China

来源：

FRONTIERS IN ONCOLOGY | 2020年 / 10卷

基金：

中国国家自然科学基金;

关键词：

RNA sequencing; bulk RNAseq; LCM-RNAseq; single-cell RNAseq; digital spatial profiling; spatial transcriptomics; fourth-generation RNAseq; next generation sequencing; GENE-EXPRESSION; CANCER; KINASE; CELLS; ANNOTATION; GENERATION; PROMISE; RISK;

D O I：

10.3389/fonc.2020.00447

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

RNA sequencing (RNAseq) is one of the most commonly used techniques in life sciences, and has been widely used in cancer research, drug development, and cancer diagnosis and prognosis. Driven by various biological and technical questions, the techniques of RNAseq have progressed rapidly from bulk RNAseq, laser-captured micro-dissected RNAseq, and single-cell RNAseq to digital spatial RNA profiling, spatial transcriptomics, and direct in situ sequencing. These different technologies have their unique strengths, weaknesses, and suitable applications in the field of clinical oncology. To guide cancer researchers to select the most appropriate RNAseq technique for their biological questions, we will discuss each of these technologies, technical features, and clinical applications in cancer. We will help cancer researchers to understand the key differences of these RNAseq technologies and their optimal applications.

引用

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