IgG4 inhibits peanut-induced basophil and mast cell activation in peanut-tolerant children sensitized to peanut major allergens

被引：215

作者：

Santos, Alexandra F. ^{[1
,2
,3
,4
,5
]}

James, Louisa K. ^{[2
,3
,6
]}

Bahnson, Henry T.

Shamji, Mohammed H. ^{[2
,3
,7
,8
]}

Couto-Francisco, Natalia C. ^{[1
,2
,3
]}

Islam, Sabita ^{[9
]}

Houghton, Sally ^{[10
]}

Clark, Andrew T. ^{[11
]}

Stephens, Alick ^{[1
,2
,3
]}

Turcanu, Victor ^{[1
,2
,3
]}

Durham, Stephen R. ^{[2
,3
,7
,8
]}

Gould, Hannah J. ^{[2
,3
,6
]}

Lack, Gideon ^{[1
,2
,3
]}

机构：

[1] Kings Coll London, Dept Pediat Allergy, Div Asthma Allergy & Lung Biol, London WC2R 2LS, England

[2] MRC, London, England

[3] Asthma UK Ctr Allerg Mech Asthma, London, England

[4] Coimbra Univ Hosp, Immunoallergol Dept, Coimbra, Portugal

[5] Gulbenkian Programme Adv Med Educ, Lisbon, Portugal

[6] Kings Coll London, Randall Div Cell & Mol Biophys, London WC2R 2LS, England

[7] Univ London Imperial Coll Sci Technol & Med, MRC, Allergy & Clin Immunol, London SW7 2AZ, England

[8] Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, Fac Med, Asthma UK Ctr Allerg Mech Asthma, London SW7 2AZ, England

[9] Univ Cambridge, Addenbrookes Hosp, Dept Med, Cambridge CB2 2QQ, England

[10] Pathol Partnership Cambridge Univ Hosp, Cambridge, England

[11] Cambridge Univ Hosp NHS Fdn Trust, Addenbrookes Hosp, Dept Allergy, Cambridge, England

来源：

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY | 2015年 / 135卷 / 05期

基金：

英国医学研究理事会;

关键词：

Ara h 2; basophil; basophil activation test; blocking antibodies; IgE inhibition; IgG(4); mast cells; peanut; peanut allergy; tolerance; ARA H 2; HISTAMINE-RELEASE; IGE; IMMUNOTHERAPY; DEGRANULATION; ANTIBODIES; INDUCTION; DIAGNOSIS; RESPONSES; RISK;

D O I：

10.1016/j.jaci.2015.01.012

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

Background: Most children with detectable peanut-specific IgE (P-sIgE) are not allergic to peanut. We addressed 2 non-mutually exclusive hypotheses for the discrepancy between allergy and sensitization: (1) differences in P-sIgE levels between children with peanut allergy (PA) and peanut-sensitized but tolerant (PS) children and (2) the presence of an IgE inhibitor, such as peanut-specific IgG(4) (P-sIgG(4)), in PS patients. Methods: Two hundred twenty-eight children (108 patients with PA, 77 PS patients, and 43 nonsensitized nonallergic subjects) were studied. Levels of specific IgE and IgG(4) to peanut and its components were determined. IgE-stripped basophils or a mast cell line were used in passive sensitization activation and inhibition assays. Plasma of PS subjects and patients submitted to peanut oral immunotherapy (POIT) were depleted of IgG(4) and retested in inhibition assays. Results: Basophils and mast cells sensitized with plasma from patients with PA but not PS patients showed dose-dependent activation in response to peanut. Levels of sIgE to peanut and its components could only partially explain differences in clinical reactivity between patients with PA and PS patients. P-sIgG(4) levels (P = .023) and P-sIgG(4)/P-sIgE (P < .001), Ara h 1-sIgG(4)/Ara h 1-sIgE (P = .050), Ara h 2-sIgG(4)/Ara h 2-sIgE (P = .004), and Ara h 3-sIgG(4)/Ara h 3-sIgE (P = .016) ratios were greater in PS children compared with those in children with PA. Peanut-induced activation was inhibited in the presence of plasma from PS children with detectable P-sIgG(4) levels and POIT but not from nonsensitized nonallergic children. Depletion of IgG(4) from plasma of children with PS (and POIT) sensitized to Ara h 1 to Ara h 3 partially restored peanutinduced mast cell activation (P = .007). Conclusions: Differences in sIgE levels and allergen specificity could not justify the clinical phenotype in all children with PA and PS children. Blocking IgG(4) antibodies provide an additional explanation for the absence of clinical reactivity in PS patients sensitized to major peanut allergens.

引用

页码：1249 / 1256

页数：8

共 37 条

[21] Relevance of Ara h1, Ara h2 and Ara h3 in peanut-allergic patients, as determined by immunoglobulin E Western blotting, basophil-histamine release and intracutaneous testing: Ara h2 is the most important peanut allergen [J].