Huperzine A and donepezil protect rat pheochromocytoma cells against oxygen-glucose deprivation

被引：70

作者：

Zhou, J ^{[1
]}

Fu, Y ^{[1
]}

Tang, XC ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China

来源：

NEUROSCIENCE LETTERS | 2001年 / 306卷 / 1-2期

关键词：

huperzine A; donepezil; acetylcholinesterase inhibitor; free radicals; PC12; cells; lactate;

D O I：

10.1016/S0304-3940(01)01855-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Huperzine A (HupA) and donepezil, two novel selective acetylcholinesterase inhibitors available for Alzheimer's disease, were tested for their ability to alleviate injury from oxygen-glucose deprivation (OGD) in the rat pheochromocytoma line PC12 cells. OGD for 30 min triggered death in more than 50% of cells, along with major changes in morphology and biochemistry including elevated levels of lipid peroxide, superoxide disamutase activity and lactate. Cells pretreated for 2 h with HupA or donepezil showed improved survival and reduced biochemical and morphologic signs of toxicity (statistically significant over the range from 10 muM down to 1.0 and 0.1 muM, respectively). Our results indicated that HupA and donepezil protected PC12 cells against OGD-induced toxicity, most likely by alleviating disturbances of oxidative and energy metabolism. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：53 / 56

页数：4

共 20 条

[1] NGF PROTECTS PC12 CELLS AGAINST ISCHEMIA BY A MECHANISM THAT REQUIRES THE N-KINASE [J].

BONIECE, IR ;

WAGNER, JA .

JOURNAL OF NEUROSCIENCE RESEARCH, 1995, 40 (01) :1-9

[2] INCREASED LIPID-PEROXIDATION IN VULNERABLE BRAIN-REGIONS AFTER TRANSIENT FOREBRAIN ISCHEMIA IN RATS [J].

BROMONT, C ;

MARIE, C ;

BRALET, J .

STROKE, 1989, 20 (07) :918-924

[3] Huperzine A, a novel promising acetylcholinesterase inhibitor [J].

Cheng, DH ;

Ren, H ;

Tang, XC .

NEUROREPORT, 1996, 8 (01) :97-101

[4]

Giacobini E, 2000, ANN NY ACAD SCI, V920, P321

[5] THE NEUROCHEMISTRY OF VASCULAR DEMENTIA [J].

GOTTFRIES, CG ;

BLENNOW, K ;

KARLSSON, I ;

WALLIN, A .

DEMENTIA, 1994, 5 (3-4) :163-167

[6] Protection against ischemic injury in primary cultured astrocytes of mouse cerebral cortex by bis(7)-tacrine, a novel anti-Alzheimer's agent [J].

Han, YF ;

Wu, DC ;

Xiao, XA ;

Chen, PMY ;

Chung, W ;

Lee, NTK ;

Pang, YP ;

Carlier, PR .

NEUROSCIENCE LETTERS, 2000, 288 (02) :95-98

[7] RE-EXAMINATION AND FURTHER DEVELOPMENT OF A PRECISE AND RAPID DYE METHOD FOR MEASURING CELL-GROWTH CELL KILL [J].

HANSEN, MB ;

NIELSEN, SE ;

BERG, K .

JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 119 (02) :203-210

[8] IMMUNOHISTOCHEMICAL LOCALIZATION OF SUPEROXIDE-DISMUTASE IN THE HIPPOCAMPUS FOLLOWING ISCHEMIA IN A GERBIL MODEL OF ISCHEMIC TOLERANCE [J].

KATO, H ;

KOGURE, K ;

ARAKI, T ;

LIU, XH ;

KATO, K ;

ITOYAMA, Y .

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1995, 15 (01) :60-70

[9]

MARBACH EP, 1967, CLIN CHEM, V13, P314

[10]

MCCORD JM, 1969, J BIOL CHEM, V244, P6049

← 1 2 →