Determination of alkaloids from the colchicine family by reversed-phase high-performance liquid chromatography

A reversed-phase HPLC gradient method is described to analyze some alkaloids from genus colchicum, with particular reference to the thio series members; at the same time the reliability of the results has been assessed, by calculating the main validation parameters. Moreover a short outline of the UV spectral features of the involved molecules explains the criteria adopted to investigate the presence of some minor related substances in the samples to be analyzed. (C) 1998 Elsevier Science B.V. All rights reserved.